微孢子虫基因组分析.ppt

Preliminary analysis of genome of nosema bombycis潘国庆 周泽扬 向仲怀 It is just the beginning of the big job… --The key lab of Sericulture of Agricultural Ministry, Southwest Agricultural University, Chongqing, P.R.China,400716. microsporidia First recognized in 1857,till now, has been 147 years. The first genome was published 2001,which is Encephalitozoon cuniculi, with 2.9 Mb . Found in from protists to inverbrates and all class of vertebrate. More than 140 genera and almost 1200 species recognized. Characteristic of the phylum Exist only as spores outside the host cell Invasion of host cell involves eversion of polar tube. No mitochondria and typical Golgi apparatus,and their ribosome are not of typical eukaryotic type but resemble the ribosome of prokaryotic organisms. The big question for evolutionary biologists An important problem to resolve in order to determine the phylogenetic position of microsporidia is whether they are primitive amitochondrial , having split from other eukaryotes before the origin of the mitochondria, or secondarily amitochondrial, being derived from ancestors with mitochondria. But we are more than it More researcher invest their energy into the microsporidia, especially the genus Encephalitozoon, which is related to human diseases. Our purpose is divided into two parts, one is for basic research, to discover the relationship between the Nb and silkworm, Nb and Ec; the other is that we are eager to find some key genes useful to prevent the Nb disease. background Nosema bombycis is the first important pathogen in silkworm feeding. Nosema bombycis test is time energy-consuming in silkworm feeding. Little information avaliable Proteinsentriesin NCBI Nb purification with Percoll N.b全基因组shotgun测序文库构建 1. Bascalling ( Phred and Phd2fasta) 2. Vector Mark (crossmatch) 3. Assemble (Phrap) 4. Finishing (consed) 5. Repeat Mark, ORF Prediction, Gene Annotation (Glimmer , Blastx , Blastn, Clastalw , tRNAsc