笛鲷属鱼类视蛋白基因的进化与表达研究-study on the evolution and expression of opsin gene in snapper fishes.docxVIP
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笛鲷属鱼类视蛋白基因的进化与表达研究-study on the evolution and expression of opsin gene in snapper fishes
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笛鲷属鱼类视蛋白基因的进化与表达分析
摘 要
笛鲷属(Lutjanus)鱼类隶属于鲈形目(Perciformes),广泛分布于热带和亚热带, 是我国南海海区捕捞和养殖的重要对象。本文从视蛋白基因的分子系统进化、基因序 列分析及表达分析三个方面来阐述笛鲷属鱼类的种系发生过程、视蛋白基因序列结构 及其表达情况。主要结果如下:
获得 10 种笛鲷属鱼类(红鳍笛鲷(Lutjanus erythopterus)、千年笛鲷(L. sebae)、 勒氏笛鲷(L. russellii)、约氏笛鲷(L. johnii)、金带笛鲷(L. fulvus)、金焰笛鲷(L. fulviflamma )、 画 眉 笛 鲷 ( L. vitta )、 奥 氏 笛 鲷 ( L. ophuysenii )、 紫 红 笛 鲷 ( L. argentimaculatus)、星点笛鲷( L. stellatus))的 RH(Rhodopsin)、SWS1(Short wavelength-sensitive pigment)和 SWS2a(SWS1-like pigment)基因部分区段,并构 建分子系统进化树,结合 COⅠ条形码数据,分析笛鲷属鱼类的种系发生历程。三种 视蛋白基因构建的系统进化分析表明画眉笛鲷和奥氏笛鲷,红鳍笛鲷和千年笛鲷具有 紧密的亲缘关系。此外,在 RH、SWS1 和
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