胎儿安方对no合成阻滞孕鼠胎盘凋亡基因fasfasl表达的影响-effects of fetal anfang on the expression of fasfa sl, a apoptosis gene in placenta of pregnant rats blocked by no synthesis.docxVIP

TaieranfangNOsynthesisblockadeonapoptoticgeneplacentainFas/FasLexpressionAbstractObjective:Intrauterinegrowthretardationinratsfromplacentallabyrinthlayer,basallayeranddeciduallayer,trophoblastcellsinpathologicalchangesandapoptosisregulatorygenes(Fas/FasL)proteinexpressionandotherpointofviewofTaieranFangintrauterinegrowthretardationonthelargesideratsanditsmechanism.Methods:48SDfemaleratstodeterminethepregnancyblockrandomizedinto6groups(controlgroup,modelgroup,Low-dosegroup,mediumandhighdosegroup,westernmedicinegroup),8ineachgroup.Eachgroupexceptforthecontrolgroupinthefirst14-22daysofpregnancy,intraperitonealinjectionofnitricoxidesynthase(Nos)blockerNomega-Nitro-L-arginine（L-NANE),intrauterinegrowthretardationcausedbyrats,overthesameperiodcorrespondingtreatmentfordrugtestingofpregnantmicebodyweightperdayduringpregnancy,Caesareansection22daysofpregnancythefetus,theplacenta/uterus,theweightofeachgroupwasdetectedfetal,placentalweight,fetalAngolaperfusionobservedontheliverandplacentaofpregnantmicepromotetheprotectionandgrowth,immunohistochemistry(SABC)detectplacentaltrophoblastFas,FasLexpressionStainedbyHEmorphologicalchangesofplacenta.Results:①Comparedwiththeinterventiongroup,modelgroupsignificantlyreducedfetalbodyweight(p0.01),thenoseofarumplengthwassignificantlyshorter(p0.01),significantlyreducedfetalweightcoefficient(p0.01)②modelofpathologicaldamageinplacentaltheperformancelevelofthebasallabyrinthlayerandshowedpatchynecrosisandinfiltrationofplasmacellsandlymphocytes,lostwithsinusoidreductiondecidualayerrenderedastrophoblasticproliferationandtheinterventiongroup,theperformanceofnormalplacenta.③placentaltrophoblastFas,FasLexpressioninfetalAngoladosegroupandmodelgroupweresignificantlydifferent(p0.01),andthedifferencebetweenthecontrolgroup(p0.05).CONCLUSION:①TaieranFangfortreatingthesideoftheplacentainFGRgrowthanddevelopmentofasignificantroleinthepromotionandprotectionofsafetyandside②TaieranFangfortreatingFGRplacentadamage,inhibitapoptosisofplacentaltrophoblastcells,andpr