n乙酰半胱氨酸对高氧致新生大鼠肺损伤中ros pjnk及细胞凋亡影响-effect of n - acetylcysteine on ros pjnk and apoptosis in hyperoxia-induced lung injury in neonatal rats.docx

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n乙酰半胱氨酸对高氧致新生大鼠肺损伤中ros pjnk及细胞凋亡影响-effect of n - acetylcysteine on ros pjnk and apoptosis in hyperoxia-induced lung injury in neonatal rats.docx

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n乙酰半胱氨酸对高氧致新生大鼠肺损伤中ros pjnk及细胞凋亡影响-effect of n - acetylcysteine on ros pjnk and apoptosis in hyperoxia-induced lung injury in neonatal rats

摘要病理可见肺泡间膈增宽，充血水肿，炎症反应，肺组织结构紊乱等。2.高氧暴露后新生大鼠肺组织中ROS、p-JNK蛋白表达及细胞凋亡增加，提示ROS-JNK-细胞凋亡通路可能是高氧致新生大鼠肺损伤原因之一。3.高氧暴露组新生大鼠肺组织中ROS、p-JNK及凋亡指数两两相关性分析均成正相关，提示ROS、p-JNK和细胞凋亡可能在高氧致新生大鼠肺损伤中起协同作用。4.NAC可能通过抑制ROS的产生，减少p-JNK蛋白的表达，最终减轻细胞凋亡，从而发挥对高氧致新生大鼠肺损伤的保护作用。关键词：高氧；肺损伤；N-乙酰半胱氨酸；ROS；p-JNK；细胞凋亡；ABSTRACTObjectives：Toobservethepathologicalchangesoflungtissueofneonatalratsafterhyperoxiaexposureaccordingtotheratmodelofhyperoxia-inducedlunginjuryOzdemir[1]established.TostudytheexpressionoftheROS、p-JNK、apoptosisinneonatalrats’lungtissueafterhyperoxiaexposureandtheroleofROS、p-JNK、apoptosisinhyperoxia-inducedlunginjury..TostudytheeffectofN-acetylcysteineonexpressionoftheROS、p-JNK、apoptosisinneonatalrats’lungtissueafterhyperoxiaexposure,andtoexploreitspossiblemechanismtoprovideexperimentalevidenceforbecomingdrugofearlyoftreatmentofneonatalhyperoxia-inducedlunginjury.Methods:Experimentalgrouping:Totally72SDratsofthreedaysoldwererandomlydividedinto3groups(n=24ineachgroup):airandnormalsaline(aircontrolgroup),hyperoxiaandnormalsalinegroup(hyperoxiaexposuregroup),NACandnormalsalinegroup(NACinterventiongroup).Modelestablishmentanddrugintervention:ThehyperoxiaexposuregroupandNACinterventiongroupwereplacedinhighoxygencontainerwhichcontains100%oxygen.Theaircontrolgroupwasplacedintheairinthesameroom.TheNACinterventiongroupwasinjectedofNAC(150mg/kg)throughabdominalcavityfromthefirstdaytothefourteenthday.Theaircontrolgroupandthehyperoxiaexposuregroupwereinjectedatthesamevolumeofnormalsalinethroughabdominalcavityatthesametimepoint.Samplecollectionandmeasuretarget:Eightneonatalratsweredecapitatedandremovedlungtissuefromeachgroupat3day,7day,and14dayoftheexperiment.TherightlungtissuewasplacedinasmallamountofPBS,andthenfrozeninliquidnitrogentodetecttheconcentrationofROSbyElisa.Theleftlungtissuewasfirstplacedintheneutralformaldehydeandthenparaffin-embeddedforHEstaining,immunohistochemistryandTUNEL.ThepathologicalchangesoflungtissuewereobservedbyHEstaining.Theexpressionofp-JNKproteinwasdetectedbyimmunohistochemis