raav2介导gdnf联合早期康复训练治疗大鼠脊髓损伤的实验分析-experimental analysis of raa v2 - mediated gdnf combined with early rehabilitation training in the treatment of spinal cord injury in rats.docxVIP

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raav2介导gdnf联合早期康复训练治疗大鼠脊髓损伤的实验分析-experimental analysis of raa v2 - mediated gdnf combined with early rehabilitation training in the treatment of spinal cord injury in rats.docx

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raav2介导gdnf联合早期康复训练治疗大鼠脊髓损伤的实验分析-experimental analysis of raa v2 - mediated gdnf combined with early rehabilitation training in the treatment of spinal cord injury in rats

PAGE PAGE VI rAAV2 介导 GDNF 联合早期康复训练治疗大鼠脊髓损伤的实验研究英文摘要 Experimental research on acute spinal cord injury in rats by GDNF mediated by rAAV2 in combination with the early rehabilitative treatment Abstract Objective: Choose male SD rats as the experimental subject, use GDNF mediated by rAAV2 joint early early rehabilitation training on acute spinal cord injury in rats after the injury, adopt the kinematics scores, histomorphology and immunohistochemical techniques, compared the effects on SCI of early rehabilitative treatment, gene therapy and combined therapy through analysising the changes of the rats’ movement functionat at different time, the expression of GDNF in the injured region, the changes of motor neurons’ form and amount, etc. Methods: This experiment adopted GDNF mediated by rAAV2 gene therapy, early rehabilitative treatment and combined therapy for SCI: 90 male SD rats, divided into sham-operated group(n=18), the natural healing group (n=18), gene therapy group(n=18), rehabilitation training group(n=18), combined therapy group (n=18), randomly. Each group based in accordance with different time points were randomly divided into 7d group(n=6), 14d group(n=6), 21d group(n=6). Inject rAAV2-GDNF-GFP after 24 hours for the gene therapy group and the combined therapy group. For rehabilitation training group and combined therapy group，after the success of the third day began to early rehabilitative training. The methods and indicators, (i)Evaluating the SCI rats’ movement function through the BBB score and inclined plate experiment. (ii)Observating the changes of spinal motoneuron’and neurogliocyte’ form and amount by HE dyeing. (iii)Observating the efficiency of rAAV2-GDNF-GFP infected the injured spinal cord by fluorescence microscope. (iv)Detecting GDNF antigen distribution in injured spinal cord tissue and the expression of GDNF by SABC kit. Results: (i)Kinematics scores display that the effect of rehabilitation training on 英文摘要 rAAV2 介导 GDNF 联合早期康复训练治疗大鼠