rhodococcus sp y104产几丁质脱乙酰酶的分离纯化-isolation and purification of chitinase produced by rhodocus sp y104.docxVIP

山东轻工业学院硕士学位论文山东轻工业学院硕士学位论文IIABSTRACTChitin deacetylase (CDA, E.C.3.2.1.41) is an enzyme that catalyzes the hydrolysis of acetamine groups of N-acetyl-D-glucosamine in chitin, convering it to chitosan. Chitin deacetylase producting chitosan from chitin don’t pollute the environment, and product the specific chitosan, and so on. The research of CDA aroused much interesting. We researched the CDA from Rhodococcus sp, optimized the fermentation medium and purified the CDA in the paper.The strain Rhodococcus sp. was selected from the soil, which was preserved in our laboratory. In order to enhance