生物科学论文--SSR分子标记在核桃遗传分析中的应用（可编辑）.doc

学号 XXXXXXXXX 医学院毕业设计 题目SSR分子标记在核桃遗传分析中的应用 SSR分子标记在核桃遗传分析中的应用 摘 要 目的 利用SSR技术对黑核桃元丰实生核桃云新核桃泰山野核桃和陕西实生核桃进行种质资源的遗传分析 方法 改良的CTAB法提取基因组DNA在建立了良好的PCR反应体系基础上利用筛选出来的8对引物对30份材料进行PCR扩增及聚丙烯酰胺凝胶电泳聚类分析 结果 在相似系数0682时可将供试的30份材料分为4个类群SSR groups简称SgSgⅠ类包括12份材料占供试材料的4000SgⅡⅢⅣ类各包括核桃材料的6份分别占2000SgⅠ类先和SgⅡ类群聚到一起再和其他三类聚到一起当相似系数为0793-0863之间时又可将SgⅠ类群分为2个亚群SgⅠ-1和SgⅠ-2SgⅠ-1中包括所有的元丰核桃实生后代SgⅠ-2中包括所有的陕西核桃品种SgⅡ包括所有的云新核桃品种由于采集的样本来源较近所有的材料可能是同一种品种SgⅢⅣ分别包括所有的泰山野核桃和黑核桃而且遗传复杂性高 结论 元丰实生后代陕西核桃与云新核桃遗传关系最近云新核桃遗传相似系数为1可能取材来自同一种样本泰山野核桃和以上三者有较大的差别遗传关系较远黑核桃是遗传特性差别更大最后才相聚 关键词 SSR Simple Sequence Repeat 核桃遗传分析 SSR molecular markers in walnut genetic analysis Abstract Purpose This Experiment use of this technology on the black walnut seedling Yuanfeng Yunxin Taishan wild walnut and Shanxi walnut in Genetic analysis of germplasm resources MethodsExtracting genomic DNA by Modified CTAB method In the establishment of a sound base on PCR reaction system Using of screening out of eight pairs of primers in 30 materials amplified PCR and acrylamide gel electrophoresisthen Cluster Analysis Results 30 materials fall into 4 Groups SSR-PCR Sg SgⅠconsists of 12 materials which account for 4000Every species of SgⅡⅢⅣ consists of 6 materials that account for 2000 SgⅠand SgⅡget together first then get together with the rest species SgⅠfalls into 2 subgroups that is SgⅠ-1 and SgⅠ-2 SgⅠ-1 consist of all descendants of Yuanfeng walnut SgⅠ-2 consist of all species of Shanxi walnut SgⅡconsist of all species of Yunxin walnut Because of the source of the collection of swatch is close Maybe all the materials of Yunxin walnut are the same variety SgⅢ consist of all theTanshan wild walnut and SgⅣ consist of all the black walnut The two species of all the materialscomplexity are high in heredity ConclusionsThe genetic relationshipsamong Yuanfeng Seedling generationsShanxi walnut and Yunxin walnut Genetic similarity coefficient of Yunxin walnut is 1the drawn maybe from the same sampleThat are differences betw