scr技术即刻构建高成骨活性骨移植材料的实验分析-experimental analysis of scr technology for immediate construction of bone graft material with high osteogenic activity.docxVIP

Empirical study on building high osteogenic activity bone graft materials instantly by Selective cell retention technologyAbstractBackground: Bone defects, which are caused by some factors such as high energy trauma, large bone tumor resection, and severe bone infection are common, and the demands for bone graft material about artificial prosthetic replacement and spinal fusion are also very great. Autologous bone graft is still the gold standard of bone transplantation, but it increases the surgical procedure and causes many new problems such as the chronic pain, limited bone etc. In addition, autogenous bone graft does not apply to the elderly, minors and so on. Local injection of autologous red bone marrow in the treatment of nonunion and bone defect have made some success. But it requires a large amount of bone marrow, and leads to the loss of effective ingredients of bone. Therefore, now it is seldom used in clinical work. Tissue engineering bone to be built with Autologous cells in vitro by conventional amplification is an ideal bone repair materials. It is not only meeting the requirements of the cell mass, but also avoiding the ethical and moral limits. The disadvantage is that amplification of autologous cells in vitro often requires twice operations: the collection of autologous cells in vitro and returning to the areas. It spends about two weeks in vitro for amplification and more cost for the treatment. There are still some difficulties to apply it for clinical works for its high level of technique. Selective cell retention technology(SCR) is reported as one of enriching technology of bone marrow by scholars of home and aboard in recent years. SCR technology may enrich effective ingredients such as stem cells and bone factor by the good adhesion and the appropriate mesh structure of enriched material by negative pressure of device when the bone marrow through them, thus it is easy to build a appreciate enriching material immediately. SCR technology has o