甘薯长喙壳病菌对三种寄主的致病性与ITS分析（可编辑）.doc

甘薯长喙壳病菌对三种寄主的致病性与ITS分析 5 10 15 20 25 30 云南农业大学生物多样性与病害控制教育部重点实验室昆明 650201 摘要已有研究表明甘薯长喙壳菌具有明显的寄主专化性本试验为了探明甘薯长喙壳菌对 石榴甘薯和芋头三种寄主的致病性分别用针刺浸根接种方法进行了原寄主交互接种 同时进行 rDNA-ITS 序列分析结果表明从三种寄主上分离的病原物都对原寄主有致病性 甘薯分离物对石榴和芋头均不能侵染表现出明显的寄主专化性然而石榴和芋头分离物 则能够相互侵染为此对石榴甘薯芋头的分离物进行 ITS 扩增得到 600bp 左右片段 经测序并在 NCBI 上进行 BLAST 比对发现石榴菌株与芋头菌株的同源性为 100而与 甘薯菌株的同源性为 97通过 DNAMAN 对该六个菌株进行同源序列比较发现石榴与 芋头菌株的碱基差异较少而甘薯与石榴和芋头菌株的碱基差异较大 关键词甘薯长喙壳菌致病性ITS-PCR 中图分类号S43663 Pathogenicity and ITS Sequence Analysis of Ceratocystis fimbriata to Three hosts Sui Wei Li Xiufeng Li Jie Huang Qiong Key Laboratory of Bio-diversity for Plant Diseases Management of Minisyry of Education Yunnan Agricultural University KunMing 650201 Abstract It was recorded that Ceratocystis fimbriata was a host-specializationThis experiment aims to study pathogenicity of Ceratocystis fimbriata on pomegranate sweet potato and taro with pricking and dipping root method to originalcross host inoculation And do rDNA-ITS sequence analysis as well The results showed that the isolates have the pathogenicity to their three own host respectively The isolates from sweet potato can not infect that pomegranate and taro with host ?specialization However The isolates from pomegranate and taro can infect each other The ITS internal transcription spacer primer pair ITS1 and ITS4 were used and amplified the fragment around 600bp Analysis of the ITS sequence data showed that the isolates were 100 homologous between taro and pomegranate isolates but 97 homologous from sweet potato to both taro and pomegranate isolates by BLAST analysis The comparison results by DNAMAN showed that the taro and pomegranate isolates almost the same more difference between sweet potato to taro and pomegranate isolates Keywords Ceratocystis fimbriata pathogenicity ITS-PCR 35 0 引言 甘薯长喙壳菌 Ceratocystis fimbriata Ellis Halst 隶属子囊菌门核菌纲球壳目 长喙 壳科是一种在双子叶植物上寄主范围广泛的真菌病原物一些易感病的植物种类有悬铃木 [1-5] 症状有木质部变色萎蔫溃疡和 40 根部腐烂近年来由该菌引起的石榴枯萎病出现并在短期内造成石榴植株大片枯死严 重影响了石榴生产和发展造成了巨大的经济损失 由于甘薯长喙壳菌具有明显的寄主专化性为