_葡萄糖苷酶水解银杏黄酮糖苷的研究.pdfVIP

安徽农业科学 ,Journal of Anhui Agri. Sci. 2008 ,36( 1) :30 - 32 　　　　　　　　　　　　　　　　　　　　　责任编辑 　刘月娟 　责任校对 　马君叶 β 葡萄糖苷酶水解银杏黄酮糖苷的研究 1 1 ,2 伍 毅 ,王洪新 　 ( 1. 食品科学与技术国家重点实验室 ,江南大学食品学院 ,江苏无锡 214012 ;2. 石河子大学食品学院 ,新疆石河子 832003) β ( ) 摘要　采用 葡萄糖苷酶水解银杏叶提取物 GBE ,使糖苷型黄酮转化为苷元型黄酮 。通过正交试验得出水解的最佳工艺参数 ,即温度 40 ℃,酶浓度 5 ×10 - 3 mg/ ml ,pH 值5. 0 下水解6 h 。由HPLC 图谱可得该条件下水解的苷元得率9. 08 % ,纯度68. 24 % 。酶解产物中还部 分保留了银杏内酯等活性成分 ,有利于保留银杏叶提取物的综合生物活性 。 β 关键词　银杏叶提取物 ;黄酮苷元; 葡萄糖苷酶;水解 ( ) 中图分类号　Q946 　　文献标识码　A 　　文章编号　0517 - 6611 2008 01 - 00030 - 03 β Study on Hydrolyzing Ginkgo Flavone Glycoside with glycosidase ( State Key Laboratory of Food Science and Technology , School of Food Science Technology , Southern Yangtze University , Wuxi , Jiangsu WU Yi et al 　 214012) β Abstract 　 glycosidase was used to hydrolyze the extract from ginkgo leaves to transform flavone glycoside into flavone aglycone. The optimum techno logical parameters of hydrolysis were obtained through orthogonal experiment , which were enzyme concentration of 5 ×10 - 3 mg/ ml and pH value of 5. 0 , hydrolysis temperature of 40 ℃and time of 6 h. It was known from HPLC spectrogram that the aglycone yield of hydrolysis under this condition was 9. 08 % with purity of 68. 24 %. In the enzymolysis products , the active ingredients such as ginkgo lactone were also reserved partly , which was in favor of reserving the synthesized bioactivity of ginkgo leaves extract. β Key words 　 Ginkgo leaves extract ; Flavone aglycone ; glycosidase ; Hydrolysis ( ) [5] 　　银杏 Ginkgo biloba L. 属银杏科银杏属多