基于胶体金和量子点免疫层析技术的分析及应用-analysis and application of immunochromatographic technique based on colloidal gold and quantum dots.docx

下载文档 降价啦

19
0
约5.86万字
约 74页
2018-06-05 发布于上海
举报
版权申诉
保障服务

基于胶体金和量子点免疫层析技术的分析及应用-analysis and application of immunochromatographic technique based on colloidal gold and quantum dots.docx

1、本文档共74页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。

基于胶体金和量子点免疫层析技术的分析及应用-analysis and application of immunochromatographic technique based on colloidal gold and quantum dots

基于胶体金和量子点免疫层析技术的研究及应用英文摘要Developmentandapplicationofcolloidalgoldandquantumdots-basedimmunochromatographicassaysAbstractImmunochromatographicassay(ICA)hasbecomeanimportantmonitoringtechniqueinfoodsafetycontrolbecauseitisasimple,fast,lowcostandon-sitedetection.Inthisthesis,twokindsofprobes,e.g.colloidalgoldnanoparticles(Au)andquantumdots(QDs)werepreparedandcharacterized.Theprobeswerelabeledwiththeantibody(Ab)todevelopbothquantumdots-basedICAstripsforthedetectionofmelamineandcolloidalgold-basedICAstripsforthedetectionoffuraltadonemetabolite(5-methylmorpholine-3-amino-2-oxazolidinone,AMOZ)andmelamineinfoodandfeedsamples.Themainresearchworksweresummarizedasfollows:Colloidalgoldofthesizeabout18nmwaspreparedusingtrisodiumcitrateasreducingagent.ThecolloidalgoldwascoupledwithAMOZmonoclonalantibodiestoformAu-Abconjugate.Antigenandrabbitanti-mouseIgGwererespectivelycoatedonnitrocellulosemembraneasthetest(T)lineandcontrol(C)line.Thecolloidalgold-basedimmunochromatographic(Au-ICA)stripsconsistingofsamplepad,Au-Abconjugatepad,adsorptionpad,etc.wereassembled.ThevisualdetectionlimitoftheAu-ICAforAMOZwasfoundtobe10ng/mLandthedetectiontimewasabout10min.FoodandfeedsamplesspikedwithAMOZweredetectedbyAu-ICAstripsandconfirmedbycorrespondingenzyme-linkedimmunosorbentassay(ELISA).TheresultsobtainedbytheproposedassaywereinagoodagreementwiththoseofELISA.ColloidalgoldwascoupledwithmelaminemonoclonalantibodyforformAu-Abconjugate.TheAu-ICAstripsformelaminewereassembeldwiththesimilarwaylikeAMOZstrips.Thevisualdetectionlimitofmelaminestripswasfoundtobe10ng/mLandthemeasurementwascompletedwithin10min.Themelaminestripscouldbeusedfor150daysat4℃withoutsignificantlossofactivity.Thedetectionresultsofthestripsforspikedsamplesmilk,milkpowderandanimalfeedswereingoodcorrelationwiththoseofELISA.Usingmercaptopropionicacidasastabilizer,water-solubleCdTequantumdots英文摘要基于胶体金和量子点免疫层析技术的研究及应用(QDs)wereprepared.BycoatingZnSonthesurfaceofCdTe,CdTe/ZnScore/shellquantumdotswereobtained.Afterpurification,theCdTe/ZnSqua