玉米气生根早期发育的转录组和候选基因的功能研究-study on the function of transcriptome and candidate genes in the early development of aerial rooting in maize.docxVIP

玉米气生根早期发育的转录组和候选基因的功能分析comparingthebraceroottranscriptomewiththatofmaizeprimaryrootindicatedsubstantialdifferencesincategoriesandabundancesofexpressedtranscripts,indicatingthefunctionandtheregulationmechanismofthebracerootisobviouslydifferentfromthemechanismoftheprimaryroot.Then,15candidategeneswereselectedtobeheterologouslyoverexpressedinArabidopsis.Wemainlyobservetherootsystemphenotypesoftransgenelinesforpreliminaryfunctionresearch.Sofar,ofalltheArabidopsisoverexpressionlinesobtained,overexpressionofZmCCD(Zeamayscorticalcell-delineatingprotein)lineswereobservedwiththesuppressedprimaryrootelongation.Furthermore,thepheynotypeofshorterprimaryrootof35S::CCDcanberecoveredunderhighconcentrationofauxin,indicatingthatZmCCDmayplayaroleintheregulationofmaizerootorbracerootearlydevelopmentbyauxin.Inconclusion,wefirstlyrevealsthecomplexchangesinthetranscriptomeduringtheearlydevelopmentofmaizebracerootandprovidesacomprehensivesetofdataessentialforunderstandingitsmolecularregulations.What’smore,wehavecharacterizedsomecandidategeneswhichtakepartintheregulationofbracerootsinitiation.Keywords:maize;braceroot;Solexasequencing;tag;differentialexpression;heterologouslyoverexpression4符号说明ABA:Abscisicacid，脱落酸Amp:Ampicillin，氨苄青霉素BR:Brassinosteroid，油菜素内酯CaMV35S:35SpromoterofCaliflowerMosoicVirus，花椰病毒35S启动子cDNA:ComplementaryDNA，互补DNACTK:Cytokinin，细胞分裂素DEPC:Diethylpyrocarbonate，焦碳酸二乙酯dNTP:Deocyribonucleosidetriphosphate，脱氧核糖核苷三磷酸EDTA:Ethylenediaminetetraaceticacid，乙二胺四乙酸EST:Expressedsequencetag，表达序列标签Eth:Ethylene，乙烯GUS:β-glucuronidase，β-葡糖醛酸酶Kan:Kanamycin，卡那霉素LB:Luria-bertanimedium，LB培养基MiRNA:microRNA，微小RNAMOPS:3-(N-morphplino)propanesulfonic，3-N吗啉代丙磺酸MS:MurashigeandSkoogmedium，MS培养基N:Nodetissues，玉米茎节NR:Nodetissueswithjust-emergedbraceroots，刚突出气生根的茎节QC:Quiescentcenter，静止中心qRT-PCR:Quantitativereal-timePCR，荧光定量PCRRNaseA:RibonucleaseA，核糖核酸酶ARpm:Revolutionsperminute，转/分钟RT-PCR:ReversetranscriptativePCR，反转录PCRSAGE:SerialAnalysisOfGeneExpression，基因表达系列分析SDS:Sodiumdodecylsulfate，十二烷基硫酸钠TE:Tris.HC