Oct4在多种人腺癌组织中表达水平研究.docVIP

Oct4在多种人腺癌组织中表达水平研究 　　[摘要] 目的：研究5种人腺癌中Oct4的表达情况，为进一步探索其在肿瘤发生中的机制提供基础。方法：应用乳腺癌、胃癌、胰腺癌、肠癌、子宫内膜癌5种腺癌的组织芯片，通过免疫组织化学染色研究Oct4蛋白在组织中的表达情况，并与癌旁正常组织进行比较。结果：Oct4在5种腺癌肿瘤组织内均有表达，胃癌组织内Oct4的表达高于癌旁正常组织（P 　　[关键词] Oct4；腺癌；组织芯片；免疫组织化学 　　[中图分类号] R730.2 [文献标识码]A[文章编号]1674-4721（2011）03（b）-０17－０4 　　 　　Research the expression of Oct4 in multiple adenocarcinoma 　　ZHANG Jinlong1 , WANG Haijuan2, LU Zhong1, LIU Jian2, LIN Chen2, QIAN Haili2*, LI Guixin1* 　　1.Weifang Medical University, Weifang 261053, China; 2.State Key Laboratory of Molecular Oncology, Cancer Institute/Cancer Hospital, Chinese Academy of Medical Sciences Peking Union Medical College, Beijing 100021, China 　　[Abstract] Objective: To study the expression of Oct4 in adenocarcinomas from 5 tissue origins and provide insights to the Oct4-related mechanism of carcinogensis. Methods: Immuno-histochemistry staining was adopted to study the expression of Oct4 protein in adenocarcinoma tissue array containing adenocarcinoma and paratumoral samples of 5 tissue origins. Results: Oct4 was expressed in all 5 adenocarcinomas. Oct4 was significantly differently expressed in gastric-derived adenocarcinoma and paratumoral tissue (P 　　1.3.2 免疫组织化学评分Oct4在细胞核、细胞浆中均有表达，分别评分。评分标准如文献[4]所述，按阳性细胞数分为：阳性细胞数75%为4分。按染色强度分为：基本未着色、着色与背景相似者为0分；着色浅略高于背景者为1分；中度着色、明显高于背景者为2分；强染、呈深棕色者为3分。对每一组织点来说，用阳性细胞数评分与染色强度的乘积为最终评分，0～4分为弱表达，5～8分为中等表达，9～12分为强表达。综合染色强度和细胞数量进行分级，取表达最强的部分。 　　1.4 统计学分析 　　所得数据采用SPSS 16.0软件进行分析，差异采用非参数检验，相关性分析采用秩相关，P 　　 　　[参考文献] 　　[1]Hochedlinger K, Yamada Y, Beard C, et al. Ectopic expression of OCT-4 blocks progenitor-cell differentiation and causes dysplasia in epithelial tissues [J]. Cell,2005,121(3):465-477. 　　[2]Gidekel S, Pizov G, Bergman Y, et al. Oct-3/4 is a dose-dependent oncogenic fate determinant [J]. Cancer Cell,2003,4:361-370. 　　[3]Chen YC, Hsu HS, Chen YW, et al. Oct-4 expression maintained cancer stem-like properties in lung cancer-derived CD133-positive cells [J]. PLoS ONE,2008,3(7):e263