一种脱细胞真皮基质制备方法研究.docVIP

一种脱细胞真皮基质制备方法研究 　　[摘要]目的：研究脱细胞真皮基质（acellular dermal matrix，ADM）的一种新的制备方法。方法：取大鼠背部皮肤制成厚度0.8mm的脱毛皮片，置入1mol/L NaCl溶液中24h，揭去表皮层，再浸入0.5%Triton X-100溶液中室温震荡48h，得到ADM。取标本行组织学观察，并行异体移植实验。结果：NaCl-Triton法制作的ADM，细胞成分脱除彻底，胶原纤维排列规则，没有明显的免疫原性，生物相容性好。结论：采用NaCl-Triton法制备ADM,价廉而有效，利于推广应用。 　　[关键词]脱细胞真皮基质，制备 　　[中图分类号]Q813[文献标识码]A[文章编号]1008-6455（2008）08-1176-02 　　 　　Research to a preparation method of acellular dermal matrix 　　MAO Yu-long1,LV Ji-zhong2,SONG Bing1 　　(1.Department of Oral and Maxillofacial Surgery, Binzhou Medical Collage Affiliated Hospital, Binzhou 256603, Shandong, China; 2.Department of Stomatology, Heze Medical Collage) 　　 　　Abstract：ObjectiveTo research a new method of producing acellular dermal matrix (ADM). MethodsThe non-hair skin grafts with 0.8mm in thickness were acquired from the back of Wistar rats. Then the grafts were treated with 1M NaCl 4℃ for about 24 hours to rip away the cuticular layers, and the remained dermis were immerged into 0.5%Triton X-100 shaking in normal temperature for about 48 hours to obtain ADM. Took samples of the ADM to do histology observation, and homoplastic tronsplantation.ResultsThere was no cell constituent remained in ADM produced by NaCl-Triton method, and the collagen fibers were in a regular arrangement. ADM had very low immunocompetence and good biocompatibility.ConclusionUsing NaCl-Triton method to produce ADM was low-cost and effective, so it was advantageous to populize application. 　　Key words：acellular dermal matrix; preparation 　　 　　皮肤或粘膜缺损是口腔颌面外科常见病症。自体移植是最佳方式，但常面临皮源不足的制约，故研究真皮替代物具有重要意义。脱细胞真皮基质（acellular dermal matrix，ADM）源于天然皮肤，动物实验和初期临床应用已证明其为理想的真皮替代物[1-2]。ADM的制备方法不一，结合文献[3-4]，我们探讨了利用NaCl-Triton法制备ADM的可行性。 　　 　　1材料和方法 　　 　　1.1实验动物：健康成年雌性Wistar大鼠46只（购于山东大学实验动???中心，合格证号SCXR(鲁，体重(250±25)g，随机分为供皮组（10只）和异体埋植组（36只）。 　　1.2 试剂与仪器：TritonX-100（美国amresco公司产品）；SCW-CJ-1B(1BU)型超净工作台（苏州宏瑞净化科技有限公司）；SGQ-250A鼓式自锁取皮机（上海医疗器械有限公司手术器械厂）；IMAGE-PROPLUS病理图像分析系统（美国Media Cybernetics公司）。 　　1.3 实验步骤 　　1.3.1取皮：大鼠脱