不同区域脑组织对运动诱导氧化应激和DNA损伤反应.docVIP

不同区域脑组织对运动诱导氧化应激和DNA损伤反应 　　摘要：目的：观察8周规律游泳运动对脑组织不同区域的氧化应激体系和DNA损伤的影响。方法：符合实验条件的24只 雄性SD 大鼠随机分为两组：安静组、递增负荷8周规律游泳组。采用免疫组化染色和生物化学检测方法，观察8周规律游泳运动对脑组织不同区域丙二醛（MDA）含量和谷胱甘肽过氧化物酶（GSH-PX）活性以及8-羟基-2’-脱氧鸟苷（8-OHdG）表达情况的影响。结果：8周规律游泳可以使大鼠海马组织的GSH-PX活性增加，同时MDA含量降低（p 　　关键词：运动；8-羟基-2’-脱氧鸟苷；氧化应激；皮层；海马 　　中图分类号：G804.2 文献标识码：A 文章编号：1007-3612(2010)12-0051-03 　　Different Response in Rat Special Brain Regions to the Oxidative Stress and DNA Damage Triggered by Exercises 　　TANG Guang?xu?1，ZHAO Li?2, LV Yuan?yuan?2, LI Yan?2, HE Ling?xiao?2 　　(1.Department of Physical Education, Hexi University, Zhangye 734000, Gansu China; 　　2.Beijing Sport University, Beijing 100084 China) 　　Abstract:Objective：To investigate the effects of 8 weeks swimming training on oxidative stress system and DNA damage in different brain region in rats. Method: The male SD rats were divided into the following categories:Twenty?four rats were placed in a tank for swimming twice a day for 90min, in total 8 weeks each. And rat special brain levels of MDA and GPx were measured. Furthermore, the expression of 8?OHdG was detected with immunohistochemical method. Result: 8 weeks swimming training resulted in increased GPx activity in the rat hippocampus accompanied by significant decrease in baseline MDA levels. Interestingly, there was no significant difference in the baseline MDA levels and the GPx values in rat cortex. Meanwhile, the comparison of 8?hydroxy?2?deoxyguanosine (8?OHdG) in both the hippocampus and the cortex did not reveal any significant differences. Conclusion：This swimming protocol appears to increase the activity of GPx only in the hippocampus, while it does not happen in the cortex. Although exercise training affects the activity of GPx in hippocampus and cortex differently, it does not increase DNA damage in the cortex. These data provide evidence for differential regulation dependent on brain region in oxidative challenge?associated adaptation during exercise. 　　Key words: exercise； 8?OHdG； oxida