中小强度有氧运动后大鼠右心室肌蛋白质组差异表达研究.docVIP

中小强度有氧运动后大鼠右心室肌蛋白质组差异表达研究 　　摘 要: 目的:运用蛋白质组学的理论和方法来研究中小强度有氧运动对大鼠右心室肌蛋白质 组的影响。方法:将20只SD雄性大鼠随机分 为对照组和运动组(各1组,每组n=10)。采用中小强度运动负荷跑台训练建立运动实验模 型,提取右心室肌组织全蛋白样品,采用双向凝胶电泳进行蛋白样品分离。运用ImageMaste r 2D Platinum图像分析软件对2-DE胶进行分析,通过图像分析,选取运动后差异表达量≥5 倍的蛋白点,用串联飞行时间质谱蛋白质仪(ULGRAFL-FLEX-TOF/TOF)进行质谱鉴定。结果 :经过8周运动后,在2-DE图谱上运动组和对照组检测到的蛋白质点分别为(547±18)个和(5 30±14)个,匹配率分别为(71.34±3.25)%和(70.28±2.38)%。运动后共有58个蛋白质 点发生了变化:表达量上调≥2倍的点有33个,下调≥2倍的点 有25个,其中上调≥5倍的点有8个,下调≥5倍的点有8个。选择差异表达量≥5倍的蛋白质 点为目标蛋白质进行质谱分析,共鉴定出了5个差异表达蛋白质点。结论:在长期中小强度 有氧运动后,大鼠右室肌蛋白质组发生了明显的差异性表达,这些差异性表达的蛋白质点为 进一步观察在运动应激状态下对心脏结构与功能影响的新的目标蛋白质提供了实验依据。 　　关键词:蛋白质组;右心室肌;中小强度有氧运动;双向凝胶电泳;质谱鉴 定 　　中图分类号:G804.2文献标识码:A文章编 号:1007-3612(2010)10-0048-04 　　 　　The Research of Different Expressions of Right Ventricular Muscl e Proteome after Moderate and 　　Small Intensity Aerobic Exercise in Rats 　　XU Zhe1,SHI Shao-Rong1,TAN Jun2,GUO Yuan-Pan1,WANGJuan1 　　(1 Physical Department of Hunan Normal University, Changsha 4 10082,Hunan China; 　　2 Peoples Hospital of Hunan Province, Changsha 41 0005, Hunan China) 　　Abstract: objective: Proteomic methodology was used to study the influence of Moderate andsmall intensity aerobic exercise on the right ventricle proteins in rats.Methods: 20 rats were divided intorest and exercise groups in random The exercise group was set up to do treadmi l l training with medium and small intensity exercise and then killed to extract t he total protein of right ventricular muscle for two dimensional gel electrohore sis searation The spots in different expression volume more than 5 times are s elected as alternative spots for mass sectra (MS) identification The gels mapswere analyzed with the ImageMaster 2D Platinum image analysis software to choos e variable proteins and the chosen proteins were identified by ULGRAFL-FLEX-TO F/TOF Results: After 8 weeks training, in electrophoresis map, the average nu mber of detected protein spot in rest group and exercise group are respectively530±14 and 547±18,and the match ratio are respe