医院病区空气真菌监测.docVIP

医院病区空气真菌监测 　　摘要 目的：研究医院病区空气中真菌浓度、菌种分布类型，为医院真菌感染的预防和治疗提供参考。方法：2007年9月1日～10月30日，对兰州某三甲医院儿科、呼吸科及血液科的治疗室、走廊、医生值班室、普通病房及监护病房进行空气真菌的分离培养。结果：儿科、呼吸科、血液科空气真菌浓度分别为263.43±184.86、72.74±33.22、101.24±57.80 CFU/m3。儿科常见的为分枝孢子菌属、曲霉属、节菱孢属、红色酵母菌；呼吸科为分枝孢子菌属、青霉属、离蠕孢属、头状地霉；血液科为青霉属、曲霉属、分枝孢子菌属、头状地霉、节菱孢属。空气真菌浓度在消毒前（分别为190.03、54.06、124.51 CFU/m3）后（分别为21.30、8.19、16.38 CFU/m3）有显著性差异（P 　　关键词 空气真菌；医院；监测 　　 　　Abstract OBJECTIVE：To supervise the airborne fungal concentration and species distribution in ward of a Certain Hospital of Lan Zhou,in order to provide a reference for the prevention and treatment of the fungal infection in hospital. METHODS：Data from 1 Sep 2007 to 30 Oct 2007 were collected from therapeutic department, corridor, duty room, public ward and intensive care unit(ICU), respectively in the department of pediatrics,the pneumology department and the hematology specialty.The finding can be obtained by separation culture to fungi. RESULTS：The airborne fungal concentration was 263.43±184.86,72.74±33.22,101.24±57.80 CFU/m3 at the department of pediatrics, the pneumology department and the hematology specialty, respectively. The most prevalent fungi collected from the department of pediatrics were Cladosporium spp, Aspregilus spp,Arthrinium spp,Rhodotorula spp in turn.The fungi collected from the Pneumology Department were Cladosporium spp, Penici l l i um spp, Aragaki spp, Geotrichum capitatum spp in turn. The fungi collected from the hematology specialty were Penici l l i um spp, Aspregilus spp, Cladosporium spp, Geotrichum capitatum spp, Arthrinium spp in turn. There was no significant difference on the airborne fungal concentration before and after sterilizing（P 3，紫外线照射60 min后（上午10时）再次将消毒的沙保罗氏培养基平皿（直径90 mm）分别置于治疗室的治疗台、医生值班室的办公桌、普通病房的床头柜上各放置2个，暴皿5min，然后关盖置于25℃恒温箱进行培育。暴皿后培养时间为3-10天??消毒前共240个培养皿；消毒后共144个培养皿。 　　1.2.3 评价方法 　　应用肉眼直观和小培养法镜下鉴定真菌，其孢子长成即进行分离鉴定，分别记录真菌种类及数量；对曲霉菌进行茶氏培养基培养后鉴定。 　　1.2.4 统计学方法 　　SPSS11.5进行均数比较( ±s)和秩和检验。 　　 　　2 结果 　　 　　2.1 三个病区五室消毒前的真菌分