子宫内膜癌中Syk基因表达及其甲基化分析.docVIP

子宫内膜癌中Syk基因表达及其甲基化分析 　　【摘要】 目的 探讨Syk基因在子宫内膜良、恶性组织中的表达及其启动子甲基化与子宫内膜癌发生、发展的关系。方法 采用RT?PCR方法检测52例子宫内膜癌及癌周组织、30例子宫内膜不典型增生、28例复杂型?生、30例单纯型增生和40例正常内膜组织中Syk mRNA 的表达情况，同时采用MSP方法检测其基因启动子甲基化情况。结果 在子宫内膜癌组、癌周组织、不典型增生组、复杂和单纯型增生及正常子宫内膜组中Syk基因表达依次增高，Syk基因甲基化程度依次降低，差异有统计学意义(P0.05)；子宫内膜癌中Syk基因的阳性表达及甲基化程度与癌症的临床分期(χ?2=4.65，P0.05;χ?2=0.94，P0.05)。Syk基因启动子甲基化与Syk基因表达缺失明显相关(P 　　【关键词】 　　子宫内膜癌；Syk基因； 启动子；甲基化 　　?? 　　 　　The expression and methylation analysis of Syk gene in endometrial cancer 　　 　　LIANG Feng,WANG Zhi?jun,MA Zhao?wen,et al. 　　Maternal and Child care service Centre in Zaozhuang City,Zaozhuang 277100,China 　　? 　　【Abstract】 Objective 　　To explorethe expression of Syk gene in benign and m alignant tissue of endometrium, the relationship between promoter methylation of those tissue and the development of endometrial cancer is also investigated. Methods Syk mRNA was detected by RT?PCR in 52 cases of endometrial cancer and adjacent tissues, 30 cases of endometrial atypical hyperplasia, 28 cases of complex hyperplasia, 30 cases of simple hyperplasia and 40 cases of normal endometrial tissue. Methylation of Syk promoter in all cases were detected by MSP methods. Results Follwing the groups of endometrial cancer, adjacent cancer tissue, endometrial atypical hyperplasia, complex hyperplasia, simple hyperplasia and normal endometrial tissue, expression of Syk gene was increased and promoter methylation was decreased.There was a significant difference (P0.05;χ?2=0.94，P0.05). Promoter methylation of Syk gene was positively correlated loss expression of Sky gene.Conclusion Promoter methylation of Syk gene may be one reason for inactivation of it, which may cause the occurrence and development of endometrial cancer.? 　　【Key words】 　　Endometrial cancer; Syk gene; Promoter; Methylation 　　? 　　 　　? 　　作者单位：277100山东省枣庄市妇幼保健医院 　　 　　 　　 　　子宫内膜癌是女性生殖道常见的三大恶性肿瘤之一，发病率呈明显增高和低龄化趋势1，2］，发病原因及机制尚未完全阐明。近年来，脾酪氨酸激酶基因(spleen tyrosine kinase, Syk)引起了国内外学者的重视，认为Syk 基因是一种新发现的候选抑癌基因，其失活可能与某些肿瘤