人参皂苷Rb与芳维甲酸乙酯对光老化皮肤真皮成纤维细胞基质金属蛋白酶影响的比较研究_论文.docxVIP

人参皂苷Rb1与芳维甲酸乙酯对光老化皮肤真皮成纤维细胞基质金属蛋白酶影响的比较研究 作者：程基焱， 刘国红， 黄吉春， 余鸿， 刘广益 【关键词】 人参皂苷Rb1；,,芳维甲酸乙酯；,,基质金属蛋白酶；,,成纤维细胞；,,免疫组织化学 摘要：目的通过人参皂苷Rb1与芳维甲酸乙酯对皮肤真皮体外培养光老化成纤维细胞胞质内基质金属蛋白酶表达的影响，阐明人参皂苷Rb1对光老化的防治机理，探索光老化的防治手段。方法建立体外培养真皮成纤维细胞光老化模型，用人参皂苷Rb1与芳维甲酸乙酯进行干扰，免疫组织化学技术显示基质金属蛋白酶的表达的改变。结果人参皂苷Rb1与芳维甲酸乙酯对培养成纤维细胞胞质基质金属蛋白酶的表达有显著抑制作用（P)。结论人参皂苷Rb1对光老化成纤维细胞具有与芳维甲酸乙酯相似的防治作用，其机理与芳维甲酸乙酯的作用机理可能相同或者相似，及通过调节MMPs活性防治皮肤光老化。 关键词：人参皂苷Rb1； 芳维甲酸乙酯； 基质金属蛋白酶； 成纤维细胞； 免疫组织化学 Comparative Study of the Effect of the Ginsenoside Rb1 and Arotinoid Ethyl Ester on the Expression of MMPs in Sun-induced Aging Dermis Fibroblasts Abstract：ObjectiveTo explore the methods of prevention and cure of sun-induced aging dermis, and clarify the mechanism of Ginsenoside Rb1 on sun-induced aging dermis, with the study of expression of matrix metallo preteinases in sun-induced aging dermis fibroblasts cytoplast, which are cultured in vitro, under the effect of Ginsenoside Rb1 and arotinoid ethyl ester. MethodsBuild the dermis fibroblasts sun-induced aging model, and affect the cells with Ginsenoside Rb1 and arotinoid ethyl ester, and display the change of expression of matrix metallo preteinases with immunohistochemistry. ResultsGinsenoside Rb1 and arotinoid ethyl ester had significant inhibition effect on the expression of matrix metallo preteinases in the fibroblasts cytoplast cultured in vitro（P). ConclusionGinsenoside Rb1 has effect of prevention and cure of sun-induced aging dermiss fibroblasts like the arotinoid ethyl ester, and its mechanism is the same as or like the arotinoid ethyl ester through regulating the expression of matrix metallo preteinases. Key words：Ginsenoside Rb1; Arotinoid ethyl ester; Matrix metallo preteinases; Fibroblast; Immunohistochemistry 皮肤老化是人体衰老的主要表现之一，也是目前人们极为关注的话题。长期反复的紫外线照射是皮肤光老化的重要因素，皱纹形成是皮肤光老化的最为重要特征。研究皱纹形成机理对防治紫外线辐射损伤及皮肤老化有重要意义。维甲酸对光老化皮肤的治疗作用已经被大量的研究所证实，并且已经应用于临床，但有研究表明维甲酸虽然已经经历了几代，仍然有许多很难克服的毒副作用［1~3］。人参具有保护机体细胞和全身免受应激的影响，降低血糖和血胆固醇，促进新陈代谢等功能，目前已经广泛应用于临床的许多方面