吉马酮抑制肝癌bel7402细胞增殖机制初步分析-preliminary analysis of gemma ketone inhibiting proliferation of hepatocellular carcinoma bel 7402 cells.docxVIP

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吉马酮抑制肝癌bel7402细胞增殖机制初步分析-preliminary analysis of gemma ketone inhibiting proliferation of hepatocellular carcinoma bel 7402 cells.docx

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吉马酮抑制肝癌bel7402细胞增殖机制初步分析-preliminary analysis of gemma ketone inhibiting proliferation of hepatocellular carcinoma bel 7402 cells

AbstractRhizoma curcuma, a valuable Chinese traditional medicine, has been massively applied into clinical therapy in Chinese medicine because of its anti-inflammatory, hepatoprotective, anti-bacterial, anti-thrombotic and anti-tumor properties. Germacrone is a kind of sesquiterpene extracted from R. curcuma, and has been reported to possess plenty of pharmacological properties while the anti-cancer mechanism has not thoroughly understood yet. The present study is to clarify the effect of germacrone on Bel7402 through MTT, Flow Cytometry, Western Blotting and so on. And the main research results are as follows.（1）Germacrone selectively inhibited the proliferation of Bel7402 cells in dose-dependentmanner while exhibited no obvious time-dependent tendency. The MTT assay results showed higher germacrone concentration caused higher growth inhibitory rate of Bel7402 cells. And there was no statistically significant difference between treatments for 24 h and 48 h. IC50 for 24 h treatment with germacrone was 160 μM. Furthermore, the same treatments on normal human liver cell LO2 cells revealed little side-effects.（2）Germacrone dose-dependently induced the apoptosis of Bel7402 cells and raised itsoxidative damage. By means of the Hochest staining and Flow cytometry assay, we found that higher concentration of drug led to the more obvious inhibitory effect. In addition, the Western Blotting results demonstrated that germacrone induced the expression of anti-cancer gene P53 and Bax while repressed the expression of anti-apoptosis gene Bcl-2. Furthermore, germacrone efficiently up-regulated the ROS level in Bel7402 cells. All the results exhibited that germacrone had the potential to promote the apoptosis and involved in the oxidative damage in Bel7402 cells.（3）Germacrone induced the apoptosis of Bel7402 cells through the JAK2/STAT3 signalpathway. We set several different treatments on Bel7402 cells by using germacrone and IL-6, the agonist of JAK2/STAT3 signal pathway, and th