家蚕储存蛋白storage proteinsp3基因克隆及调节黑化作用的研究-cloning of storage protein sp3 gene and its regulation of blackening in silkworm, bombyx mori.docxVIP

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家蚕储存蛋白storage proteinsp3基因克隆及调节黑化作用的研究-cloning of storage protein sp3 gene and its regulation of blackening in silkworm, bombyx mori.docx

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家蚕储存蛋白storage proteinsp3基因克隆及调节黑化作用的研究-cloning of storage protein sp3 gene and its regulation of blackening in silkworm, bombyx mori

II II 中文摘要家蚕储存蛋白 Storage protein（SP3）基因克隆及调节黑化作用的研究 BmBGRPB2，BmPPO2，BmDDC）在 siRNA 片段干扰后的表达水平，结果显示， BmPPAE，Bmserpin-3 的表达量增加，BmBGRP1，BmBGRPB2 的表达则下降，BmPPO2， BmDDC 的表达量略下降。关键词：家蚕；sp3 基因；黑化作用；RNAi 作者：何渐鸣指导老师：许雅香 PAGE PAGE IV Cloning Storage protein (SP3) gene from Bombyx mori and researching SP3 regulate melanization reaction Abstract Cloning Storage protein (SP3) gene from Bombyx mori and researching SP3 regulate melanization reaction Abstract Storage protein (SP) is storage protein in Bombyx mori .Usually synthesis in fat body of larvae and then released into the blood lymph.Taking silkworm as material, the Storage protein gene(sp3)of silkworm utilizing cDNA derived from fat body of silkworm as template was cloned and analyzed the characteristics of amino acid sequence. compared with other insectsamino acid sequence，tested the sp3 genes on the distribution of the age of 4 and 5 instars silkworm three days in the fat body, hemolymph ,midgut, silk glands,testis,ovary,epidermis,tested the sp3 genes on participating melanization reaction induced by exogenous siRNA，the main results were as follows: cloning and sequence analysis of sp3 from Bombyx mori Sp3 was amplified by PCR utilizing cDNA derived from fat body of silkworm as template. The sequence analysis displayed that the open reading frame of Bmsp3 gene with a length of 2091 bp coding 696 amino acids; with a predicted signal peptide consisting of the first 27-74 residues . The calculated molecular mass and isoelectric point of the mature protein were 82.10 kDa and 5.90, respectively. The nucleic acid and amino acid sequence of Bmsp3 was aligned with other previously identified insect storage protein,such as Manduca sexta, Helicoverpa armigera,Papilio xuthus,the results showed that the nucleic acid sequence alignment homology is above 75%,and Bombyx mori sp3 mature protein revealed the highest similarity with amino acid sequence of them. As with beta-aryl storage protein subunits homology was