减量训练对大鼠骨骼肌pi3kakt表达的影响-effect of reduced training on pi 3 kakt expression in skeletal muscle of rats.docxVIP
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减量训练对大鼠骨骼肌pi3kakt表达的影响-effect of reduced training on pi 3 kakt expression in skeletal muscle of rats
(4)ComparedwithgroupC4,TheproteinexpressionofPI3Kp85of gastrocnemiusmuscleingroupE4highlysignificantly increased(p0.01)andtheproteinexpressionofPI3Kp110αsignificantly increased(p0.05).Aktproteinandphos-GSK3β(Ser9)trendedto increase.Conclusions:Heavyloadtraininginhibitrat’sbodyweightandthePI3K/Akt/ GSK3β pathway of skeletal muscle;TheproteinexpressionofPI3Kp110α、PI3Kp85andAktofskeletal musclewaseffectivelyelevatedbyreducingtraining,andthemuscle protein synthesis was improved.Theovercompensationperiodofreducingtrainingmaybeinthreeto six days after the training.Key words: reducing training;Akt;PI3K; GSK3β论文随机验证编号:BT5431225377VI目录1前言...................................................................................................-3-1.1 选题依据................................................- 3 -1.2 研究内容................................................- 3 -1.3 研究技术路线............................................- 4 -1.4 预期结果................................................- 4 -2文献综述................................................................................................53研究方法..............................................................................................133.1 研究对象、分组及训练方案...................................133.1.1研究对象及分组..................................................................................................133.1.2训练方案..............................................................................................................133.2 取材和前处理...............................................153.3 测试指标及测试方法.........................................153.3.1肌肉匀浆制备......................................................................................................153.3.2总蛋白浓度测定..................................................................................................153.3.3WesternBlot 法测蛋白表达和磷酸化表达163.4 数据的统计学分析...........................................173.5 试验中主要试剂及仪器.......................................183.5.1主要试剂...................................
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