基质鲎试剂盒使用说明书(含偶氮)[]（Matrix limulus kit use instructions (including azo) []）.docVIP

基质鲎试剂盒使用说明书(含偶氮)[1]（Matrix limulus kit use instructions (including azo) [1]） Description of the use of color matrix limulus kit (end color method, azo - azo reagent) [purpose] the Chromogenic end-point Tachypleus Amebocyte Lysate, CE TAL is used for quantitative detection of bacterial endotoxin in vitro and is forbidden to enter the body in any way. 【 principle 】 the horseshoe reagent is a freeze-dried product of lysis of the haemolytic cells of the Oriental horseshoe crabs of the horseshoe crabs It contains C factor, B factor, coagulase and coagulase. Under the right conditions Degree, pH and no interfering substance, bacterial endotoxin activates C factor, which causes a series of enzymatic reactions, The coagulase was activated to form the coagulase, and the coagulase was decomposed into the chromogenic matrix Polypeptide and yellow p-nitroaniline (pNA, lambda Max = 405nm). In a certain amount of time, pNA The amount of the product is positively correlated with the concentration of bacterial endotoxin. Accordingly, the concentration of endotoxin can be quantitatively tested. At the same time, the nitric aniline (pNA) is also used to dye the red rose red (lambda Max = 545nm), Avoid the interference of the color of the test product itself to the absorption peak of 405nm. The detection limits can be detected in two regions: 0.1 EU/ml - 1 EU/ml and 0.01 EU/ml - 0.1 EU/ml The response time T 1 and T 2 see the factory inspection report. 【 composition 】 Bacterial endotoxin work, 2 Limulus reagent, 1.7 ml/branch, 2 Color matrix, 1.7 ml/branch, 2 Azochemical reagent 1, 10 ml/branch, 2 Diazochemical agent 2, 10 ml/branch, 2 Azochemical agent 3, 10 ml/branch, 2 HCl (reaction termination agent), 50ml/bottle, 1 bottle Bacterial endotoxin test water, 50ml/bottle, 2 bottles 【 storage 】 the shade, the best 2-8 ℃, avoid light storage. [usage] 1. Materials and equipment 1.1 reagent Reagent of limulus reagent, bacterial endotoxin, color matrix, azochemical reagent 1, azochemical re