运动性血红蛋白低下大鼠红细胞膜组分和功能变化.docVIP

运动性血红蛋白低下大鼠红细胞膜组分和功能变化 　　摘 要: 目的:探讨运动性血红蛋白低下时红细胞膜组分和功能变化的规律。对象与方法:将 雄性SD大鼠60只,随机分为3组:对照组、跑台运动组、负重游泳组。经跑台或负重游泳训 练后,将血红蛋白持续下降的大鼠纳入运动性血红蛋白低下组。取血,制备红细胞悬液,测 定红细胞膜磷脂成分、带3蛋白含量、磷脂酰丝氨酸外翻率、阴离子转运功能、葡萄糖转运 功能、钠钾ATP酶活性及变形能力。结果:与对照组比较,运动性血红蛋白低下组大鼠磷脂 中脑磷脂和卵磷脂丢失较多(?p? 　　关键词:运动性血红蛋白低下;红细胞膜;磷脂;膜蛋白 　　中图分类号:G804.2文献标识码:A文章编号 :1007-3612(2010)03-0062-04 　　Alteration of Structure and Function of Red Cell Membrane in Rat s with Sports Hypochrosis 　　PAN Xiao?gui,XU Hong?wei 　　(Department of Physical Education, Huzhou normal University,Huzhou 313000,Zhejiang China) 　　Abstract: The aim of this paper was to elucidate the alterations of red cell membrane thatoccur in rats following treadmill running or forced swimming.13 of 20 rats intreadmill training group and 10 of 20 rats in forced swimming group,whose Hb co ntent decreased insistently, were subjected to sports hypochrosis group. The pr ofile of phospholipids and band 3 protein of erythrocyte membrane were i nvestigated, and the eversion rate of phosphatidylserine, transport function ofband 3 protein, glucose transporter protein, and Na?+, k?+?ATPase activity, f iltration index of red cell, were tested. Compared with control group, cephalinand lecitin of phospholipid profile in sports hypochrosis group decreased signi ficantly, but band?3 protein increased significantly. On the other hand, the e version rate of erythrocyte membrane phosphatidylserine and filtration index ofred cell increased significantly, but band 3 protein anion transport function, g lucose transporter glucose?transport function, Na?+,k?+?ATP activity decreas ed markedly. It is suggested that following inadaptable high intensity exercise , the content of membrane phospholipids are cut off, band 3 protein crosslinked,the function of membrane phospholipids and proteins weakened, which adversely i nfluence erythrocyte deformability. As the result, red cell clearance increasesand sports hypochrosis occurs. 　　Key words: sports hypochrosis; red cell membrane; membrane ph