金合金和钛合金表面粗糙度与细菌黏附比较.docVIP

金合金和钛合金表面粗糙度与细菌黏附比较 　　[摘要] 目的:研究合金的粗糙度与细菌黏附的关系。方法:将不同Ra值的钛合金与金合金试件与黏性放线菌进行体外黏附实验,采用菌落形成单位(CFU)计数法比较培养试件表面的细菌黏附量,并用SPSS 13.0软件对结果进行分析。结果:随着研抛程序的进行,两种合金试件表面粗糙度均降低,其表面细菌黏附量也随之降低;经完善抛光的两种合金表面细菌黏附量无显著性差异。结论:表面粗糙度对细菌黏附起着重要影响,冠修复时应重视抛光。 　　[关键词] 表面粗糙度;细菌黏附;金合金;钛合金 　　[中图分类号] R78[文献标识码]A [文章编号]1673-7210(2010)01(c)-022-02 　　 　　Comparison of influence of surface roughness on gold alloy and titanium alloy on adhesion bacteria 　　LI Qian, WU Feng, LI Jinlu, ZHANG Bingsheng, YANG Shenghui, TIAN Guobing 　　(Department of Prosthodontics, the Affiliated Stomatological Hospital of Shanxi Medical University, Taiyuan030001, China) 　　[Abstract] Objective: To study the correlation between surface roughness of alloys and bacteria adhesion. Methods: Definite amounts of streptococcus mutans and actinomyces viscosus were adhered onto the surface of titanium alloy and gold alloy with different roughness, then the quantification of adhered bacteria on the surface of the materials were assayed by means of colony forming unit (CFU) method. Statistical analysis was used with SPSS 13.0 software. Results: The surface roughness and amount of plaque adhesion decreased with the increase in polishing level. There was no significant difference between gold alloy and titanium alloy by polished. Conclusion: The surface roughness of alloys is important for the early adhesion of bacterial plaque, the surface of crowns should be polished as smooth as possible. 　　[Key words] Surface roughness; Bacteria adhesion; Gold alloy; Titanium alloy 　　 　　口腔修复体的表面粗糙度可直接影响口腔微生物黏附和菌斑形成,从而增加龋病及牙周病的发病率,造成修复失败。因此,修复材料的致龋能力及如何降低表面粗糙度仍受到广泛关注。本实验通过比较经过不同抛光程序的金合金和钛合金与公认的致龋菌黏性放线菌的黏附关系,为临床提供参考。 　　1 材料与方法 　　1.1 材料 　　烤瓷钛合金(美国)主要成分:镍(Ni)71%,钛(Ti)6.4%,铬(Cr)12%,钼(Mo)9%。金铂合金(美国)主要成分:金(Au)73.8%,铂(Pt)9%,银(Ag)9.2%,锌(Zn)2%,铟(In)1.5%;标准菌株黏性放线菌(A.viscosus)ATCC 19246(北京口腔医院保存);C.D.C固体和液体培养基。 　　1.2 实验设备及仪器 　　TR2000手持式粗糙度仪,厌氧培养罐,37℃恒温培养箱,超净工作台,漩涡混合器,一次性使用培养皿,无菌试管等。 　　1.3 实验方法 　　1.3.1 试件制备制作24个0.8 cm×0.8 cm×0.1 cm蜡片,将蜡片的试验面在玻璃板上压平,