银杏黄酮苷对氧化损伤内皮细胞产生NO、eNOS和ICAM-1影响.docVIP

银杏黄酮苷对氧化损伤内皮细胞产生NO、eNOS和ICAM-1影响 　　 【摘要】 目的观察银杏黄酮苷对氧化损伤的人脐静脉内皮细胞(HUVEC)产生NO、内皮型一氧化氮合酶(eNOS)和人可溶性细胞间黏附分子(ICAM-1)的影响。 方法体外培养HUVEC，传至3代后，以不同浓度的银杏黄酮苷分别作用于HUVEC，然后进行氧化损伤处理。以硝酸还原酶法测定培养液上清中的NO水平，免疫细胞化学法检测内皮细胞 eNOS的表达，ELISA法测定细胞培养液中ICAM-1的含量。 结果HUVEC在氧化损伤（H2O2 100 μmol/L，2 h）后产生NO的量显著减少( P 　　【关键词】银杏黄酮苷；人脐静脉内皮细胞；NO；一氧化氮合酶；细胞间黏附分子 　　 　　Effect of ginkgo flavone glycosides on the production of NO,eNOS and ICAM-1 in cultured human umbilical vein endothelial cells under oxidative damage 　　 　　SHI Li-hong,MA Juan,ZHANG Li-ping,et al.Weifang Medical University，Weifang 261042,China 　　 　　 【Abstract】 ObjectiveTo investigate the effects of ginkgo flavone glycosides on the production of nitric oxide(NO),endothelial nitric oxide synthase(eNOS)and intercellular adhesion molecule-1(ICAM-1) in cultured human umbilical vein endothelial ceils(HUVEC) under oxidative damage. MethodsHUVEC were collected and cultured to the third generation.The oxidative damage cell model was established by H2O2（H2O2 100 μmol/L，2 h）.The level of NO,eNOS and ICAM-1 was examined by HNO?3 reductase method,immunohistochemical method and cell ELISA method respectively. ResultsAfter HUVEC were cultured under oxidative condition for 2 hours,the production of NO and the expression of eNOS decreased,the expression of ICAM-1 increased.Ginkgo flavone glycosides could enhance the production of NO,increase the expression of eNOS and decrease the expression of ICAM-1 in HUVEC under oxidative condition. ConclusionGinkgo flavone glycosides can protect the function of endothelial cells and enhance the production of NO by activating eNOS as well as decrease the expression of ICAM-1 under oxidative damage. 　　【Key words】Ginkgo flavone glycosides;Human umbilical vein endothelial cells(HUVEC);Nitric oxide(NO);Nitric oxide synthase(NOS);Intercellular adhesion molecule-1(ICAM-1) 　　 　　银杏黄酮苷（ginkgo flavone glycosides）是从中药银杏中提取的一种生物活性成分，是银杏的主要有效成分，具有抗动脉粥样硬化、增加冠状动脉血流量、改善脑血液循环、解除平滑肌痉挛等多种药理活性，但银杏黄酮苷对心血管作用的机制并不十分清楚。本研究通过体外培养人脐静脉内皮细胞