不同添加物和浓度对铁皮石斛组织培养影响.PDFVIP

30 东农业科学 2014 年第 苑 期 不同添加物及浓度对铁皮石斛组织培养的影响 罗焕明, 邓瑞云, 刘晓津, 邱道寿, 张 蕾, 蔡时可 东省农科院作物研究所/ 东省农作物改良重点实验室袁 东 广州 510640冤 摘要 以无菌播种取得的铁皮石斛无菌苗作为试验材料袁研究 1/2 MS 培养基中添加不同浓度的添加物对铁皮 石斛的增殖尧生根的影响遥结果表明院马铃薯汁和木瓜乳有利于诱导芽的增殖袁其中又以1/2 MS +NAA 0.2 mg/L +蔗糖 30 g/L+马铃薯 200 g/L 培养基最有利于诱导芽的增殖曰香蕉乳有利于诱导生根袁其中又以 1/2 MS +NAA 0.5 mg/L+蔗 糖 30 g/L +香蕉 100 g/L 培养基最有利于诱导芽生根遥 关键词 铁皮石斛曰组织培养曰添加物 中图分类号 S567.23 文献标识码 A 文章编号 1004-874X 2014冤07-0030-03 Effects of different additives and concentrations on the tissue culture of Dendrobium officinale LUO Huan-ming, DENG Rui-yun, LIU Xiao-j in, QIU Dao-shou, ZHANG Lei, CAI Shi-ke (Crops Re searc h Ins titute ,G uangdong A cade my of A gric ultural Sc ie nces/Key Laboratory of Crops G e ne tics and Imp rov e me nt of G uangdong Prov inc e , Guangz hou 510640, China) A bstract: T he effects of different additives on proliferation and rooting culture of De ndrobium offic inale were studied. T he results showed that: potato and papaya were conductive to bud proliferation, among which the 1/2 MS +NAA 0.2 mg/L+ sucrose 30 g/L +200 potato g/L medium was the best; banana promoted the rooting induction, in w hich 1/2 MS +NAA 0.5 mg/L+ sucrose 30 g/L+Banana 100 g/L medium was the best to induce the rooting of the shoots. Key words: De ndrobium offic inale ; tissue culture; additives 铁皮石斛 De ndrobium officinale Kimura et Migo冤袁 试管苗增殖和生根的影响袁 并试图在最适的添加物基 又称黑节草袁为兰科石斛属多年生草本植物袁是我国传 础上找出最适的浓度遥 本研究有利于提高皮石斛快速 [1] 统的名贵中药材袁具有益胃生津尧滋 清热 效 遥 现代 繁殖的质量和效率袁 确保铁皮石斛资源的保持和可持 药理表明石斛具有抗肿瘤尧 抗衰老尧 增强免疫力等 续利用袁降低组培成本袁为工厂化育苗提供技术支撑遥 能