北大医学研究生课程-分子免疫学-程序性细胞死亡-陈玉英教授PPT.ppt

程序性细胞死亡及检测技术;Cell Life; Apoptosis;Section Ⅰ Apoptosis(Type-Ⅰ Pragrammed Cell Death);Apoptosis and Diseases;概述;H. Robert Horvitz （美国） 1947-;1090 –131= 959(cells);研究历程;Process of Apoptosis;;;ER pathway;Regulation of Apoptosis;Caspase Activation in apoptosis;Schematic representation of the human caspases depicting their structure and proposed function;;Caspase Substrates;Mitochondria and Apoptosis ;Bcl-2 family proteins in apoptosis; Structure of Bcl-2 family Protein;;一、形态学检测 光学显微镜，荧光显微镜，共聚焦显微镜，电镜 二、磷脂酰丝氨酸外翻分析（Annexin V法） FCM分析，荧光显微镜，共聚焦显微镜， 三、 Caspase活性的检测 We