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ELECTROCHEMICAL SCIENCE电化学科学.pdf

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Int. J. Electrochem. Sci., 5 (2010) 1422 - 1433 International Journal of ELECTROCHEMICAL SCIENCE FFT Continuous Cyclic Voltammetry Triglyceride Dual Enzyme Biosensor Based on MWCNTs-CeO2 Nanoparticles 1,2,* 2 2 2 3 1,2 M. R. Ganjali F. Faridbod , E. Nasli-Esfahani, B. Larijani , H. Rashedi ,P. Norouzi 1 Center of Excellence in Electrochemistry, University of Tehran, Tehran, Iran 2 Endocrinology and Metabolism Research Center, Tehran University of Medical Sciences, Tehran, Iran 3 Department of Chemical Engineering, Faculty of Engineering, University of Tehran, Tehran, Iran * E-Mail: ganjali@khayam.ut.ac.ir Received: 16 August 2010 / Accepted: 19 August 2010 / Published: 1 October 2010 A new electrochemical biosensor designed for the triglyceride determination based on nanomaterials. Lipase and glycerol dehydrogenase enzymes were used as sensing materials and they were immobilized on to the CeO2 nanoparticles and Multiwall cabon-nanotubes (MWCNTs) placed on a glassy carbon electrode by nafion. The detection method was based on fast Fourier transform (FFT) continuous cyclic voltammetry in a flow injection system. Under optimal detection conditions, the linear response was in the range of 1 to 100 mg/L with detection limit of 0.5 mg/L at a signal-to-noise ratio of 3. The biosensor showed an acceptable reproducibility and good stability. Keywords: Triglyceride, biosensor, nanoparticles, FFT Cyclic voltammetry