Fish Two-generation Test Guideline鱼2代.pdfVIP

Fish Life-Cycle Test Guideline 8 November 2002 DRAFT PROPOSAL FOR A NEW GUIDELINE Fish Two-generation Test Guideline INTRODUCTION 1. This guideline describes a two-generation test with fish that considers reproductive fitness in parents and offspring as an integrated measure of toxicant. It also enables measurement of a suite of histological and biochemical endpoints that allow diagnostic and definitive evaluation of endocrine disrupting chemicals (EDCs) or other types of reproductive toxicant. This guideline is intended to be applicable to the fathead minnow (Pimephales promelas), medaka (Oryzias latipes), sheepshead minnow (Cyprinodon variegatus) and zebrafish (Danio rerio). The fish two-generation test is a relatively long-term (normally 180-d or longer) assay that assesses early development, growth, and reproduction. The two-generation test described in this guideline combines elements of the recently developed short-term reproduction test (Ankley et al., 2001) and life-cycle toxicity test methods developed for fathead minnow (Benoit, 1982) and sheepshead minnow (Hansen et al., 1978). 2. The test is intended to serve as a higher tier for collecting definitive concentration-response information on adverse effects suitable for use in ecological risk assessment. Specifically, the design enables the collection of both mechanism-specific toxicity data and information concerning fecundity and viability. The former is useful as a basis for diagnosis and/or extrapolation across species, while the latter is critical to application of population models to risk assessment scenarios. 3. The two-generation test is initiated with mature male and female (P generation) fish, eggs are collect