仪器分析课程设计 效液相色谱.docx

高效液相色谱 摘要 高效液相色谱法又称“高压液相色谱”“高速液相色谱”“高分离度液相色谱” “近代柱色谱”等。高效液相色谱是色谱法的一个重要分支，以液体为流动相，采用高压输液系统。 高效液相色谱用高压输液泵将具有不同极性的单一溶剂或不同比例的混合溶剂、缓冲液等流动相泵入装有固定相的色谱柱，经进样阀注入待测样品，由流动相带入柱内，在柱内各成分被分离后，依次进入检测器进行检测，从而实现对试样的分析。这种方法已成为化学、生化、医学、工业、农业、环保、商检和法检等学科领域中重要的分离分析技术，是分析化学、生物化学和环境化学工作者手中必不可少的工具。 关键词： 液相色谱 高效液相色谱 色谱法 流动相 High-performance liquid chromatography Abstract： High-performance liquid chromatography, also known as high-pressure liquid chromatography, high-speed liquid chromatography, high-resolution liquid chromatography, Modern chromatography and so on. HPLC chromatography is an important branch of the liquid as the mobile phase, high pressure infusion system. High-performance liquid chromatography with high-pressure infusion pump with a single solvent or a different polarity, different combinations of solvents, buffers and other mobile phase pumped into the column with the stationary phase, the injection valve into the sample, the mobile phase into the column, the column the components are separated, turn into the detector for testing, in order to achieve sample analysis. This approach has become the chemistry, biochemistry, medicine, industry, agriculture, environmental protection, inspection and seizure law in important subject areas such as separation and analysis technology, analytical chemistry, biochemistry and environmental chemistry indispensable tool in the hands of workers. Key words： Liquid chromatography HPLC Chromatography Mobile phase  PAGE \* MERGEFORMAT 23 目录  TOC \o 1-3 \h \z \u  HYPERLINK \l _Toc297374533 目录  PAGEREF _Toc297374533 \h I  HYPERLINK \l _Toc297374534 第一章 概述  PAGEREF _Toc297374534 \h 1  HYPERLINK \l _Toc297374535 1.1高效液相色谱的发展历史  PAGEREF _Toc297374535 \h 1  HYPERLINK \l _Toc297374536 1.2高效液相色谱特点  PAGEREF _Toc297374536 \h 2  HYPERLINK \l _Toc297374537 1.3高效液相色谱的基本概念和术语  PAGEREF _Toc297374537 \h 3  HYPERLINK \l _Toc297374538 第二章 高效液相色谱基本理论  PAGEREF _Toc297374538 \h 6  HYPERLINK \l _Toc297374539