观察度量与纪录.PPTVIP

* Conclusion: Tenascin-C inhibits polarization and transmigration of T cells, that consequently prevents a direct contact of tumor and T cells and maintains immune suppressive status. Hwang JY, Cheng YJ, Lin YP, Lin HC, Su CC, Juliano R, and Yang BC (2010) Extracellular matrix of glioblastoma inhibits polarization and transmigration of T cells: a role of tenascin-C in immune suppression. J Immunol. 185:1450-1459. * 3-dimentional migration of leukocytes NPC (CD3 stain) HE Why T cells infiltrate and accumulate here? Is there any intrinsic difference between tissue/cells? 第二種想法 喜歡待在那裡，內在原因! * * Our in vitro 3D migration setting We purified the collagen from pig skin. Tumor spheroids are generated. Time-lapse recording. Yuhas JM, et al. (1977) Cancer Res 37:3639-3643. Culture on 0.5% Nobel Agar MCF-7 spheroid U-118MG/HE SEM of U118MG spheroid attraction repulsion The T cells final position relative to a common origen. The surface of spheroid is set as 0 of X axis/Y axis * * Lin YP, Cheng YJ, Huang JY, Lin HC, and Yang BC (2010) Zap70 controls the interaction of talin with integrin to regulate the chemotactic directionality of T cell migration. Mol Immunol. 47(11-12):2022-9. Wu CY, Tsai YY, Chen SY, Lin YP, Shin CC, Wu CC, and Yang BC (2017) Interaction of Zap70 and CXCR4 receptor at lamellipodia that determines the directionality during Jurkat T cells chemotaxis. Mol Immunol 90:245–254. * Zap70 first at the protrusion site! Images processed by ImageJ: /search/label/ImageJ 回縮 (retraction) 前撲 (focal complex extension) 定位 (focal adhesion) * * NIH3T3 cells model T cell migration * 以上的觀察程序符合哪一項? 三種方式來描述觀察的事物，有目地的！ 分類（Classification）: 黑白紅黃、金銀銅鐵、草木蟲魚是分類的語言。 量化（Quantification）: 有單位、附帶數字的是量化的語言。 比較（Comparison）: 大小、長短、高低、冷暖、新舊、強弱等可以配成對來使用的，是比較的語言。 * * Before get going the experiments, let’s review a quick history about cell migration. When cells are attracted in reaction to a chemical stimulus, they move against the chemical gradient and it is called positive che