SIMPLEX VIRUS单纯疱疹病毒课件.ppt

HERPES SIMPLEX VIRUS Characteristics of HSV DNA double stranded virus, linear 125-250 Kb long, relatively big Enveloped Virion size 200 nm, relatively big 9 HSVs, Ex. Varicella, EBV, CMV Diseases: Chickenbox, Mononucleosis, Hepatitis, Encephalitis Recurrent eye, mouth and genital lesions Herpes Virus and Common Diseases Everybody knows chickenpox and likely you experienced the disease as a child, can be dangerous when exposed to it in adulthood Another common ailment is lip and mouth “cold sores” Genital Herpes lesions caused by HSV, sexually transmitted HSV-1 cold sores (mild but annoying diseases) HSV-2 genital herpes Varicella zoster: chickenpox However the Herpes family is huge, over 100 members HSV Establishes Latent Infections Once infection has taken place HSV can remain dormant for months, years, lifetime Cell types that HSV can infect and remain dormant Neurons, B-cells and T-cells Examples: Shingles which can appear years after first chickepox infection (caused by varicella zoster, causes both chickenpox and shingles) Genital Herpes outbreaks Herpes (1-2) Simplex Virus Genome HSV Capsid Enclosed in an envelope Capsid has icosahedral structure Capsid is bilayered Constructed from 6 proteins VP5 is the main one Envelope contains at least 10 different glycoproteins gB-gM Envelope also contains non-glycosylated proteins HSV Entry Into Host Occurs Via Heparan Sulfate Proteoglycans gB and gC bind to host glycoproteins with heparan sulfate moieties (repeating dissacharide: glucoronic and n-acetyl glucosamine) Following gB and gC is gD which binds to nectin1D OR HVEM (herpes virus entry mediator) Fusion occurs between viral envelope and host membrane Nucleocapsid is released into cytosol OR in acidified endosomes Transport to nuclear envelope occurs via ?T and capsid interaction DNA is released into nucleus Capsid disintegrates Genome Expression in Nucleus Viral DNA is circularized once inside nucleus Viral DNA is localized in regions referred to as