细胞因子信传导抑制因子-3.docVIP

SOCS-3在妊娠期肝内胆汁淤积症母胎界面的表达及意义 李明月 张丽娟＊ 【摘要】目的 初步探讨细胞因子信号传导抑制因子-3（suppressor of cytokine signaling-3 ,SOCS-3）在妊娠期肝内胆汁淤积症母胎界面的表达及意义。方法 选择单胎产妇45例，其中ICP组30例为试验组，分为ICP重型组10例和轻型组20例，对照组为健康孕妇15例，无妊娠并发症与合并症。采用免疫组化的方法测量胎盘SOCS-3的表达，采用ELISA的方法检测胎盘组织匀浆IL-10及INF-γ的浓度。结果 1 SOCS-3主要表达于细胞滋养细胞和合体滋养细胞，偶见绒毛基质细胞阳性表达，散在分布于蜕膜间质内，蜕膜局部子宫血管壁可见阳性表达；2 ICP组胎盘组织SOCS-3的表达较正常妊娠组明显降低，ICP组重度者SOCS-3的表达较轻度低，两两比较差别均有统计学意义（P﹤0.01）;3 ICP组胎盘组织分泌IL-10较正常妊娠组明显降低（P﹤0.01）,而INF-γ较正常妊娠组明显升高，差别有统计学意义；4 ICP胎盘组织表达SOCS-3与胎盘组织分泌INF-γ/IL-10呈负相关性。结论 SOCS-3表达明显降低，可能参与了ICP的发病，SOCS-3降低导致ICP可能与Th1/Th2比例失衡有关。 【关键词】SOCS-3 妊娠期肝内胆汁淤积症 IL-10 INF-γ 胎盘 Expression and significance of socs-3 on placenta in Intrahepatic cholestasis of pregnancy 【Abstract】Objective:To explore the expression and significance of socs-3 on placenta in Intrahepatic cholestasis of pregnancy.Method:45 cases were selected in this study.Expression of socs-3 in placenta collected from 30 ICP patients including mild (n=20)and severe(n=10) and 15 normal later pregnancy women were assessed by immunohistochemistry analysis.At the same time, The density of IL-10 and INF-γ from placenta homogenate are measured by ELISA.Results:1 socs-3 immunostaining is mainly localized in the syncytio- trophoblast layer in the villous tissue.Sometimes in the matrix cell and vessel wall of decidua.2 socs-3 immunostaining was much reduced in the villous tissue from ICP placentas, both in preterm and term placentas, compared to those from normal placentas.3 IL-10 from placenta homogenate of ICP was much reduced, compared to those from normal placentas and it was contradictory to INF-γ.Conclusion: The depressed expression of socs3 in ICP is possible one of the pathogenesies of ICP and may relate to the ratio disequilibrium of Th1/Th2. 【Key word】SOCS-3 IL-10 INF-γ ICP placenta 细胞因子信号转导抑制因子(suppressor of cytokinesignaling,SOCS)是近年来新发现的一类负向调节蛋白,可抑制多种细胞因子的信号通路,且大多数成员可被细胞因子和生长因子所诱导产生。目前已发现8种SOCS分子,即SOCS1-7及CIS(cytokine inducible SH2 containingprotein),其中SOCS1、SOCS2、SOCS3在机体免疫调