(免疫学课件)antigen presentation and T cel responses.pptxVIP

Antigen presentation and T cell responseXuanming YangSchool of life science and biotechnologyA comparison of ligand for innate and adaptive immunityreceptorligandAntigen processing required MacrophageTLRPathogen products (LPS,CpG viral RNA)NOB cellBCRAntigen (protein or others)NOab T cell (CD8)TCRComplex of MHCI-peptide from protein antigen Yesab T cell (CD4)TCRComplex of MHCII-peptide from antigenYesT cells do not recognise native antigens+M?LPSResponse+ResponseBProtein AgYY+No ResponseProtein AgTQuestions for this lecture That T and B cells recognise antigen differently Pathway for endogenous antigen presentation to T cell (MHC-I). Pathway for exogenous antigen presentation to T cell (MHC-II). The structure of MHC-peptide TCR complexCell surface peptides of Ag presented by cells that express MHC antigensSolublenative AgSoluble peptidesof AgCell surfacepeptidesof AgCell surfacenative AgAntigens must be processed in order to be recognised by T cellsANTIGENPROCESSINGT T cellresponseNo T cellresponseNo T cellresponseNo T cellresponseNo T cellresponseAntigen processingAg recognition by T cells REQUIRES presentation by MHC on a cell membrane (MHC restriction)Pathways for Ag presentation: a) Class I MHC assoc. with peptides from endogenous Ag’s b) Class II MHC assoc with peptides from exogenous Ag’sMHC-I and MHC-II expressing cellExogenous Antigen processingExogenous Ag’s are typically phagocytized/ endocytized by M? and APC’sForeign Ag is degraded w/i endocytic vacuole of endocytic pathway. The pathway includes:Early endosomes (pH 6-6.5)Late endosomes or endolysosome (pH 5-6)Lysomes (pH 4.5 – 5)Ag is degraded into 13-18 aa polypeptides which bind to MHC IIEventually endocytic vacuole returns to PM ? MHCII-peptide presentationExogenous Antigen processingHow peptides bind to MHC-IIHow peptide bind to MHC-IIAgAPCM?M?T cellresponseWhat form of antigen is produced by antigen processing?Ovalbumin specific CD4 T cell lineNative ovalbuminDigested ovalbuminM?M?ViableViableFix