周围神经束活体术中简便快速准确无创鉴别可行性分析.docVIP

周围神经束的活体术中简便快速准确无创鉴别的可行性分析 【摘要】 目的:根据周围神经感觉束与周围神经运动束等一系列的鉴别方法探索出能够快速，准确，无创，无误，简便，能够进行活体检测的几种重要的鉴别神经功能束的方式方法。 方法：通过现有的各种鉴别的方式方法----解剖图示法、电刺激法、酶染法、免疫染色法等来探索出一些新的有关于鉴别人体体神经纤维束的方式方法。 附加：由于上述这几种方式方法无法突破所需时间比较长，步骤比较繁琐，而且不适用于陈旧性的神经纤维损伤,还有特异性较差，又无法进行活体检测等限制和缺陷，已逐渐失去了临床应用价值。 结果：通过蛋白组学筛选的方法来鉴别周围神经感觉束与周围神经运动束相关联的特异性蛋白，利用纳米量子点的独特发光的特性，还有通过试纸化筛选的方法，就可以实现快速，准确，无创，无误，简便，通过活体检查就可以进行的方法来鉴别神经功能纤维束。 结论：基础性的研究及高科技材料的联合应用，特异性分子蛋白的发现，还有荧光稳定、明亮、生物学相容性比较好的量子点探针的设备的制备以及试纸在临床上的广泛应用将为生物学方面的诊断和治疗提供既客观又直观的依据,因为这些方法可以实现快速，准确，无创，无误，简便等活体相关联的神经功能束的生物学性质的检测。 关键词：周围神经束；神经感觉束与神经运动束；蛋白质组学检查；纳米量子点 Abstract Objective: To revise the history of clinical methods to distinguish between sensory and motor nerve fascicles of the peripheral nerve system and to explore potential novel techniques in this field. Methods: The principles and shortcomings of currently used diverse methods for sensory and motor nerve fascicle identification, including nerve morphology, electric stimulation, spectroscopy, enzymohistochemistry staining (AchE, CA and ChAC histochemistry staining methods), and immunochemical methods, were systematically reviewed. The progress of diffusion tensor imaging, proteomic approaches and quantum dots in possible clinical applications to identify sensory or motor fascicles were further discussed. Results:Traditional ways including physical and enzymohistochemical methods are not suitable for the precise differentiation of sensor and motor nerve fascicles. Immunohistochemical staining using AchE, CA, and ChAC has shown promise in advancing sensory and motor nerve fascicle differentiation. Diffusion tensor imaging can reflect morphological details of nerve fibers. Proteomics reveals the dynamic selection of the specific proteins discriminating sensory and motor fascicles. Quantum dots, with their size-dependent optical properties, make them an ideal candidate for sensory and/or motor nerve protein markers. Conclusion: Diffusion tensor imaging, proteomics approach and quantum dots-imaging, due to t