Chromatographic Separation vinstan home色谱分离 vistan回家.pptVIP

Chromatographic Separation Alina Guna Ryan Young Edward Sun Jonathan Lam Mr. Vincent SCH3U3 Contents Introduction to Chromatography Adsorption Paper Chromatography Experiment Materials Procedure Observations and Calculations Video Applications Conclusion Sources of Experimental Error Introduction Chromatography – Separating Mixtures Includes a mobile/stationary phase Preparative or Analytical Preparative: Separate to use components Analytical: Measure relative proportion of a substance 5 different types of Chromatography: Adsorption Partition Ion Exchange Molecular Exclusion Affinity Adsorption Chromatography When a mobile gas or liquid collects onto a stationary solid Forms a thin layer of molecules Solvent travels up adsorbent to meet sample Continues upwards as a solvent/solute solution (adsorbate) Different compounds in sample Different solubilities Travels different distances The other types … Partition Chromatography Separation of similar substances by repeated extraction by two immiscible liquids. Ion Exchange Separation of ions and polar molecules Molecular Exclusion Particles are separated based on size. Usually applied to large molecules such as proteins and industrial polymers Affinity Method of separating biochemical mixtures We focus on Adsorption in our project. Paper Chromatography Stationary Phase Chromatography Paper Mobile Phase Unreactive solvent Primarily used to separate and identify coloured mixtures Two-way paper chromatography Complicated substances Amino Acids Solvent’s ability to move upwards via capillary action Experiment Explain adsorption What it is How it is used to separate mixtures Demonstrate adsorption through paper chromatography Calculate retention factors of the substances Safety Concerns Safety Goggles Rubbing Alcohol Spillage/Leakage Consumption Ventilation of Area Permanent Markers Condition Wastage Materials Glass Jars Chromatography Paper Pencils Rubbing alcohol (70% Isopropyl Alcohol) Sharpie Pens of