盐酸氨溴索对百草枯中毒大鼠肺SP—D表达及肺损伤保护作用.docVIP

盐酸氨溴索对百草枯中毒大鼠肺SP—D表达及肺损伤保护作用 　　【摘要】目的 观察盐酸氨溴索对百草枯中毒致急性肺损伤大鼠肺表面活性物质蛋白-D（alveolar surface active substance-D，SP-D）及各相关指标的影响，并评价盐酸氨溴索对百草枯中毒治疗作用。方法 将120只雄性大鼠，随机（随机数字法）分为3组：生理盐水组（NS组），百草枯中毒组（PQ组），盐酸氨溴索治疗组（AT组）；再分别将每组按时间点（第1、3、5、7天）随机（随机数字法）分为4组。各组以Western blot法检测肺组织SP-D蛋白含量，以HE染色观察大鼠肺组织形态学变化，并同时检测各组大鼠的白细胞计数（white blood count，WBC）和中性粒细胞（polymorphonuclear， PMN）百分比及动脉血氧分压（arterial oxygen pressure，PaO2）。结果 AT组第3、5、7天WBC计数均低于PQ组，高于NS组各相应时间点，差异有统计学意义（P2各相应时间点均低于NS组（P2高于PQ组，差异有统计学意义（P 　　【关键词】百草枯；急性肺损伤；肺泡表面活性物质相关蛋白-D；盐酸氨溴索 　　Study of the effect of ambroxol hydrochloride on pulmonary substances protein-D， WBC and PaO2 in rats toxicated with paraquat MA Yu-teng*， DU Yue-ju， ZHANG Jun-li， SHI Han-wen， TIAN Ying-ping， GAO Heng-bo， SU Jian-ling， LV Cui-huan. *Department of Emergency，The Second Affiliated Hospital of Hebei Medical University， Shijiazhuang 050000， China 　　Corresponding author： SHI Han-wen， Email：mayuteng2004@ 　　【Abstract】Objective To observe the effects of ambroxol hydrochloride （AH） on surfactant protein-D （SP-D） changes in rats with acute lung injury （ALI） from paraquat （PQ） poisoning in order to evaluate the therapeutic effect of AH.Methods One hundred and twenty male rats were randomly （random number） divided into three groups， namely NS （normal saline） group， PQ group， and AT （ambroxol treat） group. On the 1st， 3rd， 5th and 7th day after PQ poisoning， blood samples and lung tissue were taken for laboratory examination. Venous blood samples were taken to detect WBC count and percentage of PMN； and arterial blood for measuring PaO2. The rat lung tissue morphological changes were observed with HE staining， and lung tissue surfactant protein-D （SP-D） content was determined with Western blot.Results The numbers of WBCs in AT group were lower than those in PQ group and higher than those in NS group on the 3rd， 5th and 7th day （P2 in AT group were lower than those in NS group at the same intervals （P2 in AT group were higher on the 3rd， 5th and 7th day （P 　　【Key wo