过氧化物酶Ⅰ在正常和骨关节炎膝关节软骨组织中表达.docVIP

过氧化物酶Ⅰ在正常和骨关节炎膝关节软骨组织中表达 　　[摘要] 目的 探讨过氧化物酶Ⅰ在正常和骨关节炎膝关节软骨组织中的表达。 方法 选取本院2010年10月～2012年10月行单侧膝关节表面置换术的骨关节炎患者39例为观察组，选取本院同期行单侧膝关节表面置换术的非骨关节炎患者26例为对照组，分别提取部分膝关节软骨，采用Western Blot方法检测膝关节软骨组织细胞中过氧化物酶Ⅰ的表达水平。 结果 观察组平均积分光度值明显高于对照组，观察组膝关节软骨组织表层、中层、深层的氧化物酶Ⅰ免疫组化染色的平均积分光度值均明显高于对照组，差异有统计学意义（P 0.05）。观察组膝关节软骨组织表层、中层、深层的氧化物酶Ⅰ免疫组化染色的平均积分光度值呈现明显的增加趋势，相互间比较差异均有统计学意义（P 　　[关键词] 过氧化物酶Ⅰ；骨关节炎；膝关节；软骨组织；表达 　　[中图分类号] R684 [文献标识码] B [文章编号] 1673-9701（2013）32-0063-03 　　Expression of PrxⅠin normal and OA cartilage tissues of knee joint 　　WU Chao 　　Second Department of Surgery， Urumqi Petroleum Chemical General Factory Worker Hospital， Urumqi 830019， China 　　[Abstract] Objective To investigate expression of PrxⅠin normal and OA cartilage tissue of knee joint. Methods A total of 39 OA patients who treated knee arthroplasty were selected in hospital from October 2010 to October 2012 as observation group. 26 non-OA patients who treated knee arthroplasty were selected in hospital at the same period as control group. Part of knee joint cartilage were extracted. Expression of PrxⅠin cartilage tissue cells of knee joint was detected by Western blot method. Results Average integral absorbance value in observation group was significantly higher than control group. Average integral absorbance values of knee joint cartilage surface， middle， deep in observation group were significantly higher than control group. Difference was statistically significant （P 0.05）. Average integral absorbance values of knee joint cartilage surface， middle， deep in observation group showed significant increasing trend，while difference was statistically significant （P 　　1.1 一般资料 　　选择本院2010年10月～2012年10月行单侧膝关节表面置换术的OA患者39例为观察组，年龄47～69岁，平均（58.3±7.2）岁，男15例，女24例，其中左侧19例，右侧20例，手术时间72～136 min，平均（93.1±27.5）min，术后随访3个月。患者均符合OA的诊断标准，排除患有心肺疾病、肝肾疾病、血液病、精神病患者。本院同期行单侧膝关节表面置换术的非OA患者26例为对照组，年龄35～70岁，平均（56.1±10.4）岁，男10例，女16例，其中左侧13例，右侧13例，手术时间78～134 min，平均（91.8±26.7）min，术后随访3个月。排除患有膝关节疼痛病史和软骨疾病的患者。手术原因：车祸伤21例、摔倒伤5例。两组患者的基础资料（年龄、性别、患侧、手术