2013年生猪良种补贴项目供精单位汇总.ppt

* AMPK and Apoptosis Emeline Van Goethem José Manuel López Apoptosis or programmed cell death is an important part of the development of all multicellular organisms and controls homeostasis and tissue modeling. In the cell there are mitochondrial dependent and independent pathways to induce apoptosis. Mitochondria Cytochrome c Caspase activation AMPK is conserved between different species AMPK: Energy Homeostasis and Apoptosis (Blast ClustalW analysis) Consensus sequence ?(X,?)XXS/TXXX? ?: MVLIF ?: RKH Expasy Tools Scan Prosite [MVLIF]x(0,1)[RKH]xx[ST]xxx[MVLIF] FEBS Letters 361 (1995) 191-195 Dale S., et al. Target proteins for AMPK Homo sapiens 1000 Xenopus laevis 678 Xenopus laevis + mitochondria 17 VDAC2 (Voltage dependent anion channel 2) Regulates cytochrome c release / apoptosis Looking for an Apoptotic protein Regulated by AMPK AIM VDAC2 -AVPPSYADLGKSARDIFNKGYGFGLVKLDVKTKSATGVEFTTSGTSNTDSGKVNGSLET VDAC1 MAIPPAYVDLGKSARDIFTKGYGFGFIKLDLKTKSENGLEFTSSGSANAETSKVSGNLET MGC81430 MAVPPTYADLGKSARDVFNKGYGFGLVKLEVKTKSSSGVEFTSSGSSNTDTGKASGNLET VDAC2 KYKWGEYGLTFTEKWNTDNTLGTEIAIEDQIAKGLKLTFDTTFSPNTGKKSGKVKAAYKQ VDAC1 KYKWAEYGLTFTEKWNTDNTLGTEITVEDQLAKGLKLTFDSSFSPNTGKKNAKVKSAYKR MGC81430 KYKMKETGLTFTQKWNTDNTLGTEVALEDKLAKGLKLSLDTTFVPNTGKKSAKLKTSYKR VDAC2 EYVNLGCDVDFDFAGPAIHGSAVVGYEGWLAGYQMTFDSAKSKLTKNNFAVGYKTGDFQL VDAC1 EHLNVGCDMDFDIAGPSVRGAVVFGYEGWLAGYQMTFESSKSRVSQSNFAVGYKTDEFQL MGC81430 DYANLGIDIDLDLAGPTIYGWGVLGYQGWLAGYQMAFDTAKSRLAQNNFALGYTAGDFQL VDAC2 HTNVNDGSEFAGSIYQKVSDKMETAVNLAWTSGNNSTRFGIAAKYQLDSHAAISAKVNNS VDAC1 HTNVNDGTDFGGSVYQKVNDKLETAINLAWTAGNSNTRFGIAAKYQIDSDASFSAKVNNS MGC81430 HTNVNDGTEFGGSVYQKVSKDVETSVSLAWSAGSNNTRFGIGAKYQLDSNTTISAKVNNA VDAC2 SLVGVGYTQTLRPGVKLTLSALVDGKNINAGGHKLGLGLELEA VDAC1