Gas Chromatography and Mass Spectrometer uses - TeacherWeb气相色谱和质谱仪的使用teacherweb.pptVIP

Gas Chromatography and Mass Spectrometer uses Definitions: Gas Chromatograph-a machine used to separate mixtures into each component using various detectors. Mass Spectrometer-an analysis that identifies and measures the particles mass Isotope-any of two or more species of atoms of a chemical element with the same atomic number and nearly identical chemical behavior but with differing atomic mass or mass number and different physical properties GC/MS-Gas chromatography and Mass spectrometry Uses for GC/MS: Did you know that mass spectrometry is used to... Detect and identify the use of steroids in athletes Monitor the breath of patients by anesthesiologists during surgery Determine the composition of molecular species found in space Determine whether honey is adulterated with corn syrup Locate oil deposits by measuring petroleum precursors in rock Monitor fermentation processes for the biotechnology industry Detect dioxins in contaminated fish Determine gene damage from environmental causes Establish the elemental composition of semiconductor materials Check monitor wells to determine where the gas contamination came from. Was it naturally occurring from the earth or did some outside source contaminate it? More GC/MS uses: In the U.S. TV show CSI for example, GCs are used to rapidly identify unknown samples. medical, pharmacological, environmental, and law enforcement fields all use GC/MS What a GC does: Sample is prepared Sample is injected Carrier gas pushes the sample through columns—I use 3 different type of columns on the GC’s I run. Molecular Sieve, Capillary column, Hay Sep A column Sample is pushed to the detector and the components are identified. GC Info: Detectors used on our GC’s: Thermal Conductivity Detector (TCD) and Flame Ionization Detector (FID) Computer software is used to quantize the peaks from the chromatography trace. MS overview, cont. The isotope-ratio mass spectrometer (IRMS) allows the precise measurement of mixtures of st