5―羟色胺转运体与肠易激综合征患者躯体心理症状相关性分析.docVIP

5―羟色胺转运体与肠易激综合征患者躯体心理症状相关性分析 　　[摘要] 目的 研究5-羟色胺转运体（SERT）与肠易激综合征（IBS）患者躯体和心理症状是否存在相关性。 方法 选取2010年1月～2012年12月佛山市第一人民医院IBS患者154例为病例组，并按不同症状分为3个亚组（C-IBS组65例、D-IBS组47例、A-IBS组42例），选择同期健康志愿者154名作为对照组。应用心理症状自评量表（SCL-90）来评价研究对象的心理状态；采用实时定量PCR法测定SERT信使RNA（mRNA）表达量，Western印迹法测定SERT蛋白表达水平。 结果 SERT蛋白表达量：病例组（0.81±0.45）和对照组（0.79±0.47）比较，差异无统计学意义（P 0.05）。SERT mRNA转录表达量：病例组（0.46±0.25）和对照组（0.42±0.28）比较，差异无统计学意义（P 0.05）。C-IBS组SERT蛋白表达量（0.98±0.51）及mRNA表达量（0.58±0.23）均显著高于D-IBS组[（0.71±0.40）、（0.41±0.25）]、A-IBS组[（0.67±0.35）、（0.37±0.23）]和对照组[（0.79±0.47）、（0.42±0.28）]，差异均有统计学意义（P 0.05）。 结论 IBS患者SERT的mRNA及蛋白量表达水平与患者的躯体和心理症状密切相关，越低则越容易出现躯体化症状、焦虑等情绪表现。 　　[关键词] 5-羟色胺转运体；肠易激综合征；转运蛋白质；躯体化症状；心理症状 　　[中图分类号] R574.4 [文献标识码] A [文章编号] 1673-7210（2014）02（c）-0018-04 　　Correlation between expression of SERT and somatic and psychological symptoms of irritable bowel syndrome 　　LI Kejing1 QIU Xiaolei1 YUAN Yaozong2 　　1.Department of Special Medical Center， the First Peoples Hospital of Foshan City， Guangdong Province， Foshan 528000， China； 2.Department of Special Medical Center， the Affiliated Ruijin Hospital of Shanghai Jiaotong University School of Medicine， Shanghai 200025， China 　　[Abstract] Objective To explore the correlation between the expression of SERT and somatic and psychological symptoms of irritable bowel syndrome （IBS）. Methods 154 cases of IBS patients in the First Peoples Hospital of Foshan City from January 2010 to December 2012 were selected as disease group and divided into three groups （C-IBS group 65 cases， D-IBS group 47 cases， A-IBS group 42 cases） according to different symptom. 154 healthy volunteers were selected as control group. The psychological symptom of study objects were evaluated by SLC-90. The expression of SERT at mRNA level was tested by real-time potymerase chain reaction （PCR） and the expression of SERT at protein level was detected with Western blotting. Results The expressions of SERT at protein： disease group （0.81±0.45） and control group （0.79±0.47） were com