Separating and Identifying Food Dyes by Paper Chromatography纸色谱法分离和鉴定食品染料.pptVIP

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2018-06-26 发布于四川
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Separating and Identifying Food Dyes by Paper Chromatography纸色谱法分离和鉴定食品染料.ppt

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Separating and Identifying Food Dyes by Paper Chromatography纸色谱法分离和鉴定食品染料

Separating and Identifying Food Dyes by Paper Chromatography Purpose and Goals To determine Retention factors (Rf) of seven food dyes in three different solvent systems The best effective solvent of the three systems To Separate and Identify the dyes in unknown mixtures and commercial products Chromatography Group of techniques used to separate colored mixtures into their component parts Paper Chromatography Simplest form Uses Separation Identification Chromatography paper Stationary phase Solvent Mobile phase Terms Spotted Application of sample Origin line Place near bottom of paper where spotting occurs Solvent front Leading edge of mobile phase Terms Chromatogram Pattern produced in dye separation Resolved Separation of two or more components Terms Retention Factor Rf = Distance traveled by component (cm)/ distance traveled by solvent front (cm) Ranges from 0.0 to 1.0 0.0 no movement 1.0 complete movement Used to determine the best solvent Procedure I Work in groups of three Get 3 Microscope slides and petri dish covers Label three 250ml beakers and fill with 7ml Water Rubbing alcohol 10% NaCl solution Prepare and label chromatography paper Procedure I Using a separate dropper for each dye, transfer one drop each of the seven pure solutions to the three slides Label drops by placing paper with ID under the slide Use a clean wood toothpick to spot the dye to the proper spot along the origin line on the chromatography paper Procedure I Allow spot to dry before applying more dye Roll C.P. to form a cylinder with the dye spots on the outside and the origin line at the bottom Staple the ends together with the ends of the paper touching but not overlapping Procedure I Place the cylinder into the appropriately labeled beaker with the origin line at the bottom Record the time (Start time) Remove the cylinder when the solvent front is about 1.5 cm from the top and record the time (Ending time) Procedure I Carefully unroll the paper and allow to dry Do calculati