tf-spions用于大鼠脑胶质瘤mri成像的分析-analysis of tf - spions in mri of rat glioma.docx

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2018-06-28 发布于上海
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tf-spions用于大鼠脑胶质瘤mri成像的分析-analysis of tf - spions in mri of rat glioma.docx

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tf-spions用于大鼠脑胶质瘤mri成像的分析-analysis of tf - spions in mri of rat glioma

T2WI,T1WIandSWIsequenceafter2hours,24hours,48hourstoevaluatetheeffectivenessofthecontrastagent.Andthetumorsignalchangeswereobserved.Finally,thetumorbiopsyspecimenswereseparatelyperformedPrussianbluestainingandHEstainingasthebiochemicalandpathologicalstudy.(4)SPSS16.0softwarewasusedtotestthestudydatabylineindependentsamplesttest,p0.05indicatingasignificantstatisticallydifference.Results：①ThesuccessfullycouplingofTfandSPIONswasconfirmedbyFTIR.TEMshowedtheoverallsizeofSPIONswas9.3±0.5nm,Tf-SPIONswas14.1±0.6nm.Fromtheconcentration-relaxationratecurve,showingtheconcentrationofTf-SPIONsandSPIONsandT2relaxationtimewaslinear,withthesampleconcentrationincreasing,therelaxationrateincreasing.Ther2valueofSPIONswas120.4mM-1S-1accordingtoMRIimaging,andTf-SPIONswas64.3mM-1S-1.②MRIimagingrevealedthatTf-SPIONsgroupwithC6cellsinT2signalintensityismoreobviousthanSPIONsgroupinvitro.Theresultsoftheconcentration-ironcontentcurveshowedthatironcontenttheTf-SPIONsgroupswerehigherthanthecontrolinthe0-20μg/mLconcentrationrange.Prussianbluestainingconfirmedthatthenumberofpositivecellswassignificantlyhigherthanthecontrolgroup.③ThetumorsshowedasheterogenoushyperintensityonT2WIimagesandhypointensityonT1WIimaging.2haftertheinjectionofTf-SPIONs,theT2WIandSWIimagesignalstartedtodecreaseintumortissues.After24and48hoursthelowsignalareabecameobviousandthescopeincreased.However,thecontrolgroupobservednosignificantsignalchangeinthetumorarea.ThenormalbraintissuewasinvadedbygliomatumorcellsonHEstaining.PrussianbluestainingconfirmedthatlargenumberofTf-SPIONsaccumulatedintumorsite.However,rareSPIONswereaccumulated.Conclution：Tf-SPIONscouldimprovethesensitivityandspecificityofvisualizingthegliomaonT2WIandSWIimages,andcouldmaintainalongertimewindow.ItmayservedasanidealMRcontrastagentintargetinggliomainthefuture.Keywords：Glioma,Magneticresonanceimaging,Transferrin,Superpara-magneticironoxidenanoparticles独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体