乙肝病毒外膜大蛋白含量与HBVDNA载量相关性.docVIP

乙肝病毒外膜大蛋白含量与HBVDNA载量的相关性 【摘要】 目的： 探讨乙型肝炎患者血清中乙肝病毒外膜大蛋白(HBVLP)与HBVDNA水平的相关性,以及HBVLP对HBeAg阴性乙肝患者的临床应用价值。 方法： 分别采用ELISA法和实时荧光定量PCR法检测405例HBV感染者血清的HBVLP、HBeAg和HBVDNA。 结果： (1)405例HBV感染者血清中, HBVLP和HBVDNA的阳性率分别为75.06%和70.12%,两者的检出一致率为71.85%,两者间差异无统计学意义(Pgt;0.05);(2)HBVLP含量与HBVDNA拷贝数之间呈正相关(r= 0.944,Plt;0.05)。(3)HBeAg阳性158例中, HBVLP和HBVDNA的阳性检出率分别是93.67%和88.61%; HBeAg阴性247例中HBVLP和HBVDNA阳性检出率分别是63.16%和58.30%,两者间差异均无统计学意义(Pgt;0.05);(4)HBVDNA阳性284例中, HBVLP的阳性率明显高于HBeAg的阳性率,分别是83.45%和49.30%,差异有统计学意义(Plt;0.01) 。 结论： 乙肝病毒外膜大蛋白与HBVDNA两者间有很好的相关性，可以作为判断HBV复制的血清学指标。 【关键词】 乙肝病毒外膜大蛋白;HBVDNA;病毒复制 　[Abstract] Objective: To explore the correlation between the content of HBV large envelope protein(HBVLP) and the HBVDNA load in hepatitis B patients, and to discuss the clinical application of HBVLP in hepatitis B patients HBeAg negative. Methods: Serum HBVLP and HBVM were detected by using ELISA, and the HBVDNA was quantitively detected by using real time polymerase chain reaction(RTPCR). Results: (1)In 405 serum samples of the patient with HBV infection, positive rate of HBVLP and HBVDNA were 75.06% and 70.12% .(2)The level of HBVLP and HBVDNA copies performed a nice positive correlation(r= 0.944,Plt;0.05). (3) No significant difference of positive rate was found between HBVLP(93.67%) and HBVDNA(88.61%) in 158 HBeAg positive serum samples, no significant difference of positive rate was found between HBVLP(63.16%) and HBVDNA(58.30%) in 247 HBeAg negative serum samples. (4) Significant difference of positive rate was found between HBVLP(83.45%) and HBeAg (49.30%) in 284 serum samples with HBVDNA positive. Conclusion: HBVLP was a sensitive index in reflecting hepatitis B virus replication level, especially to the HBeAg negative chronic hepatitis patients. 　　[Key words] HBV large envelope protein; HBVDNA; virus replication 　　乙型病毒性肝炎是我国常见的传染病，其传染性和病死率均居高不下，严重危害人们的健康[1]。乙肝病毒血清标志物(HBVM) 检测是目前诊断乙型肝炎最常用的指标,主要反映人体对乙肝病毒(HBV) 的免疫反应状态。检测HBV 标志物只能反映病毒入侵人体的信息,不能直接反映HBV