tf spions用于大鼠脑胶质瘤mri成像的研究-application of tf spions in mri imaging of rat glioma.docx

结果:①傅里叶变换红外光谱（FT-IR）证实Tf与SPIONs偶联成功。透射电镜结果显示SPIONs粒径为9.3±0.5nm，Tf-SPIONs粒径为14.1±0.6nm。从浓度－弛豫率曲线看出，Tf-SPIONs和SPIONs的浓度和T2弛豫时间呈线性关系，随着样品浓度增大，其弛豫率也增大；根据MRI图像计算得到SPIONs的弛豫率为120.4mM-1S-1，Tf-SPIONs的弛豫率为64.3mM-1S-1。②C6细胞体外MRI成像显示，Tf-SPIONs组对C6细胞T2信号强度的改变比SPIONs组更明显。浓度—铁含量曲线结果表明，在0-20μg/mL浓度范围下试验组的细胞内铁含量均高于对照组。而且试验组普鲁士蓝染色阳性细胞百分比为（98.7±1.5）%，明显高于对照组普鲁士蓝染阳性细胞（14.5±1.2）%。③平扫荷瘤大鼠肿瘤组织呈长T1、长T2信号，注射Gd-DTPA后在T1WI上明显强化。对照组注射SPIONs后，荷瘤大鼠肿瘤区在T2加权及SWI图像上未见明显信号改变。试验组注射Tf-SPIONs后2h，荷瘤大鼠肿瘤区在T2加权及SWI图像上信号开始降低，24及48h后T2加权及SWI图像中仍可发现肿瘤区呈明显低信号，且较前范围扩大。脑组织切片行HE染色证实肿瘤细胞呈现胶质瘤细胞特征；普鲁士蓝染色证实试验组大鼠肿瘤组织内积聚大量蓝染颗粒并且位于肿瘤细胞内，而对照组肿瘤组织内几乎未见蓝染颗粒。结论:Tf-SPIONs能提高磁共振T2WI和SWI图像对于胶质瘤显示的敏感性和特异性，并且可以维持较长时间的显像，或许能作为一种较理想的靶向对比剂对C6胶质瘤进行标记成像。关键词：脑胶质瘤，磁共振成像，转铁蛋白，超顺磁性氧化铁纳米粒MRImagingofbraingliomainratmodelusingTransferrin-conjugatedSPIONsasaspecificcontrastagentDepartmentofRadiologyofUnionHospital,TongjiMedicalCollege,HuazhongUniversityofScienceandTechnologyCandidateJiangWeiliSupervisorProf.XuHaiboAbstractObjective：BytestingTf-SPIONsofratC6gliomabothinvitroandinvivoonMRIimaging,toexplorewhethertransferrin-conjugatedsuperparamagneticironoxidenanoparticles（Tf-SPIONs）couldusedasaspecificMRcontrastagentfordetectinggliomainrat.Methods：(1)TfconjuatedwithSPIONswastestingbyFouriertransforminfraredspectroscopy(FTIR).Themorphologyandparticlesizeofnanoparticleswereobservedbytransmissionelectronmicroscope(TEM).Therangeof1-20μg/mlconcentrationofTf-SPIONsandSPIONswerescannedusing3.0TMRIofT2WIsequenceandcalculatedthevalueofr2relaxationrates.(2)Thedifferentconcentrations0,5,10,15and20ug/mlofTf-SPIONsandSPIONswereincubatedwithC6gliomacellsrespectively,whichwerescannedbytheT2WIsequenceandcomparedbetweenthetwosignalstrength.Twosamplesofdifferentconcentrationsofintracellularironcontentweremeasured.Theconcentration20μg/mLofTf-SPIONsandSPIONswereseparatelyincubatedwithC6gliomacellsandthenunderwentPrussianbluestaining.(3)TheratgliomamodelwasestablishedbybrainstereotaxicinjectionofC6gliomacells.Plantedabout14days,Tf-SPIONsandSPIONswereseparately