桑椹多酚含量测定、指纹图谱和抗氧化能力研究-药物分析专业论文.docxVIP

中 文 摘 要目 的：通过对桑椹多酚类物质的含量测定方法的研究，优化桑 椹多酚提取工艺，在此基础上，建立桑椹多酚指纹图谱的研究方法， 并对桑椹多酚的总抗氧化能力进行测定，以期对桑椹多酚的开发利用 提供依据。方 法：通过对福林酚法测定总多酚含量的实验条件进行考察， 建立了桑椹总多酚含量测定方法，并通过单因素试验和 L9(34)正交试 验法，以桑椹多酚的含量为指标确定桑椹多酚的最佳提取工艺；通过 采集或购买不同产地的桑椹药材，以芦丁为质量控制指标，应用高效 液相色谱法对桑椹多酚指纹图谱的建立进行研究；采用 FRAP 法对不 同产地桑椹药材的多酚提取液进行总抗氧化能力的评估。结 果：建立了福林酚法测定桑椹总多酚含量的方法;明确了桑椹 多酚的最佳提取工艺为：50%的甲醇溶液，料液比 1:30,超声提取 30min；建立了桑椹多酚 HPLC 指纹图谱；测得各个产地桑椹多酚总 抗氧化能力在 0.685-1.523mmoL/mg 之间。结 论：福林酚法测定总多酚含量方法稳定、高效；桑椹总多酚 提取工艺效果良好，有一定的参考价值；桑椹多酚指纹图谱有一定的 稳定性和可控性，能够反映桑椹药材的内在品质。自行采集桑椹样品 抗氧化能力优于市售药材。关键词:桑椹；多酚；指纹图谱；抗氧化Mulberry polyphenol Quality evaluation and Antioxidant capacity ResearchABSTRACTObjective: Through the research of mulberry polyphenol content determination method, optimize the extractive craft of mulberry polyphenol, on this basis ， establish the fingerprint of mulberrypolyphenol and determine the total antioxidant capacity ,in order to provide a basis for the development and utilization of mulberry polyphenol.Methods: Through studying the experimental conditions of Folin-Ciocalteu method what is used for the determination of total polyphenols, to establish the determination method of total polyphenols in mulberry. through the single factor experiment and L9 (34) orthogonal test method, with mulberry polyphenol content is indicators to determine the optimum extraction technology of mulberry polyphenols; Through acquisition or purchase of mulberry of medicinal materials, different regions with rutin as index of quality control, application to mulberry polyphenol HPLC fingerprint establishment of research; FRAP method is used for different origin mulberry medicinal materials of polyphenols extract of total antioxidant capacity assessment.Results: Established the forint phenol method determining total polyphenol content in mulberry; Clearly the best extraction technology of mulberry polyphenol is: 50% of methanol solution, material liquid than 1:30, ultrasonic extraction, 30 min; Mulberry polyphenol HPLCfingerprintwasestablis