Flux-sum analysis manuscript draft通量与分析手稿.docVIP

Supplementary information Flux-sum analysis for succinate production in Escherichia coli A study on Escherichia coli strain improvement revealed that disrupting genes for pyruvate-forming enzymes leads to enhanced succinate production (Lee et al., 2005). In that study, different combinations of gene disruptions were simulated in silico and a gene knockout combination of ptsG, pykF and pykA, which increases the production of succinate by reducing the formation of pyruvate, was identified. In this case study, we demonstrate that the flux-sum attenuation analysis can arrive at the same results mo