不同生长策略典型藻种磷储存的比较分析-comparative analysis of phosphorus storage of typical algae species with different growth strategies.docxVIP

ABSTRACTAs a restrictive nutrient for life, phosphorus took widly part in many important physiological activities, such as cell division and protein synthesis. The phenomenon of phosphorus luxury adsorption appeared in many algae, they could made phosphorus stored as the form of polyphosphate (“PolyP” for short) in cell. The mechanism of phosphorus storage could be much clear by the research in the synthesis and decomposition of polyphosphates in the algae.In this paper, several detection methods of PolyP in typical algae with different growth strategies were optimized; further more, phosphorus storage characters of several typical algae were discussed.In this paper, hot-water extraction, one kind of chemical analysis methods for detection of PolyP granules in algae was founded. It was quite easy to operate. The optimal extraction temperature was made sure for algae living on different growth strategies. At the same time, fluorescence staining technology was applied in the analysis of PolyP. In this method, PolyP stained by 2-(4-amidinophenyl)- indole-6-carboxamidine dihydrochloride (DAPI) could appear yellow fluorescence when stimulated by UV. The content of DAPI was confirmed by a preexperiment. According to the result, some conclusions came as follows:① When hot-water extraction method was applied in the detection of PolyPgranules in different kind of algae living on different growth strategies, the optimal extraction temperature was different among them. The best extraction temperature for Aphanizomenon flos-aquae was 105℃, while it was 95℃ for Chlorella sp. and 125℃ for Cyclotella sp. At the same time, for these algae living in their stationary phases and cultured in the lab, the PolyP granules content in the TCP was also different from each other, it was the biggest in Aphanizomenon flos-aquae, which was 57.2%; Chlorella sp. ranked at the last by 37.2%; while Cyclotella sp. was in the middle by 53.7%.② The existential state of PolyP in algae could be