共振光散射法在核酸分析中的应用分析-application analysis of resonance light scattering method in nucleic acid analysis.docxVIP

摘要摘要IIII第四部分：苯甲醛缩水合肼钴配合物的合成及其与 ctDNA 相互作用的研究由本实验室合成金属钴配合物，并对其进行红外光谱及元素分析，确定配合物的分 子结构。采用共振光散射光谱，紫外吸收光谱，荧光光谱等多种技术研究钴配合物与 DNA 相互作用的情况及其作用方式。结果表明在 pH=5.0 的 Tris 缓冲溶液中钴配合物可 以和 ctDNA 通过静电结合的模式有效的结合成大离子的复合物，从而导致了共振光散 射强度的增强。实验并考查了 pH、离子强度、配合物浓度、缓冲溶液等对反应体系的 影响。并根据配合物的共振光散射强度在一定范围内与 ctDNA 的浓度成正比,建立了一 种简单快速测定纳克级核糖核酸的方法。该方法具有灵敏度高、线性范围较宽和操作简 便等优点。线性范围为（0.05–8.0）μg/mL；相关系数 r=0.9991，检测限为 4.31 ng/mL。关键词：共振光散射光谱法；紫外-可见吸收光谱法；ctDNA；丝裂霉素 C；酮洛芬；钴 的配合物。AbstAbstractIIIIIIAbstractIn recent years, the study of the interaction of DNA with small molecules has been become an important part in the field of chemical biology and clinical medicine. Nucleic acid is not only the fundamental materials of life, but also play an important role in the synthesis of some proteins. In addition, the nucleic acid is the basic unit which constituted organism genetic function and maintained the normal operation of various life functions. And it also plays the decisive role in the series of major biological phenomena. Nucleic acid has a very important role in the practical application, it has found that nearly two thousand genetic diseases have to do with DNA structure. Therefore, the research of the interaction between small molecules and DNA has become an important component of life science, it can be helpful in understanding the pathogenesis of certain diseases and the pharmacological mechanisms of drugs, as well as to provide a favorable information in the molecular structure and efficacy of drug. At the same time, it is also the important basis for guiding the drug designing and drug screening in vitro. In the past period of time, much attention was paid to the binding of small molecules with the DNA. In this assay, we used resonance light scattering method to study the interactions between several different types of small molecules and nucleic acid and discuss the reaction extent and mechanism.The assay is composed of four parts:Part I: The research methods and research status between the small molecules